Biolegend, 803405, PE anti-Apo E Antibody, 100tests

Apo-E is a class of apolipoprotein primarily synthesized in the liver, but is also found in other tissues such as brain, kidneys, and spleen. Apo-E is a protein component of serum chylomicron and lipoprotein particles, playing essential roles in cholesterol transportation, homeostasis, and metabolism. The expression of Apo-E is induced by cholesterol-rich diets and hyperlipoproteinemia. In the central nervous system, Apo-E is produced by astrocytes and microglia. The Apo-E-containing lipoprotein particles play important functions in brain injury repair, neurogenesis, and synaptic plasticity. Apo-E has been found to be involved in the regulation of amyloid-β aggregation and clearance. Genetic polymorphisms of Apo-E are associated with neuron degenerative disorders including Alzheimer's disease.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This antibody was raised against a synthetic peptide corresponding to amino acids surrounding the polymorphic amino acid position 158 of Apo-E.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: This antibody is effective in immunoblotting (WB). Clone E6D7 recognizes the E2, E3, and E4 isoforms of apolipoprotein E.
Application References(PubMed link indicates BioLegend citation): Munoz DG, Feldman H. 2000. CMAJ. 162(1):65-72. Hagberg JM, et al. 2000. Physiol Genomics 4(2):101-108. Saunders AM. 2000. J. Neuropathol. Exp. Neurol. 59(9):751-8. Golabek AA, et al. 2000. Biophys. J. 79(2):1008-15. Zunarelli E, et al. 2000. Breast Cancer Res. Treat. 63(3):193-8. Zunarelli E, et al. 2000. Clin. Neuropathol. 19(1):1-6. Tomiyama T, et al. 1999. Cell Mol. Life Sci. 56(3-4):268-79. Golabek AA., et al. 1999. J. Biol. Chem. 271(18):10602-6.
RRID: AB_2801139 (BioLegend Cat. No. 803404) AB_2801140 (BioLegend Cat. No. 803405)
Cell Type: Astrocytes, Mature Neurons, Microglia, Monocytes, Neurons, Oligodendrocytes
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Misfolding and Aggregation, Protein Trafficking and Clearance
Molecular Family: APP/Aβ Degradation, Apolipoproteins
Gene ID: 348
UniProt: View information about Apo E on UniProt.org
Clone: E6D7
Regulatory Status: RUO
Other Names: Apolipoprotein E, apolipoprotein E3, AD2, LDLCQ5, LPG, ApoE
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.