Biolegend, 805803, Biotin anti-mouse/rat β-Amyloid Antibody (Previously Covance catalog# SIG-39154), 50μg

Amyloid beta (Aβ or Amyloid beta) denotes peptides of 36-43 amino acids in length that are crucially involved in Alzheimer's disease as the main component of the amyloid plaques found in the brains of Alzheimer patients. The peptides result from the amyloid precursor protein (APP), which is cut by certain enzymes to yield Aβ. Aβ molecules can aggregate to form oligomers (known as "seeds") which are believed to be able to induce other Aβ molecules to also take the misfolded oligomeric form, leading to a chain reaction akin to a prion infection. The seeds or the resulting amyloid plaques are toxic to nerve cells. The other protein implicated in Alzheimer's disease, tau protein, also forms such prion-like misfolded oligomers, and there is some evidence that misfolded Aβ can induce tau to misfold.
50μg
Verified Reactivity: Mouse, Rat
Antibody Type: Polyclonal
Host Species: Rabbit
Formulation: Phosphate-buffered solution + 50% glycerol (no preservatives or carrier proteins).
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.05 mg/ml
Storage & Handling: Store between 2°C and 8°C.
Application: ELISA, WB, IHC
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. The optimal working dilution should be determined for each specific assay condition.
Application Notes: This antibody is effective in immunoblotting, immunohistochemistry (IHC), and ELISA. This antibody is specific for the rodent Aß. The rabbit anti-rodent Aß reacts extremely well with all isoforms. The rabbit anti-rodent Aß antibody has negligible cross-reactivity to human Aß using immunohistochemistry2. This product may contain other non-IgG subtypes.
Application References(PubMed link indicates BioLegend citation): Costantini C, et al. A TrkA to p75NTR molecular switch activates amyloid beta-peptide generation during aging. Biochem J 391(Pt 1):59-67, 2005. Thomas van Groen, et al. 2006. Neurobiology of Disease. 23:653. (IHC) PubMed
RRID: AB_2564690 (BioLegend Cat. No. 805803)
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family: APP/β-Amyloid
Gene ID: 351
UniProt: View information about beta-Amyloid on UniProt.org
Clone: Poly18058
Regulatory Status: RUO
Other Names: AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously: Signet Catalog# 9154-005Covance Catalog# SIG-39154
Isotype: Rabbit Polyclonal IgG
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.