Biolegend, 806002, Purified anti-APP, 573-596 Antibody (Previously Covance catalog# SIG-39180), 500microl

Amyloid beta (Aβ or Amyloid beta) denotes peptides of 36-43 amino acids in length that are crucially involved in Alzheimer's disease as the main component of the amyloid plaques found in the brains of Alzheimer patients. The peptides result from the amyloid precursor protein (APP), which is cut by certain enzymes to yield Aβ. Aβ molecules can aggregate to form oligomers (known as "seeds") which are believed to be able to induce other Aβ molecules to also take the misfolded oligomeric form, leading to a chain reaction akin to a prion infection. The seeds or the resulting amyloid plaques are toxic to nerve cells. The other protein implicated in Alzheimer's disease, tau protein, also forms such prion-like misfolded oligomers, and there is some evidence that misfolded Aβ can induce tau to misfold.
500microl
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution; no preservatives or carrier proteins.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: WB - Quality tested IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The epitope lies within amino acids 573-596 of amyloid precursor protein. Cross-reactivity to fragments may be expected based on the location of the epitope. Additional reported applications (for the relevant formats) include: immunoprecipitation2.
Application References(PubMed link indicates BioLegend citation): Halford RW and Russell DW. Reduction of cholesterol synthesis in the mouse brain does not affect amyloid formation in Alzheimer's disease, but does extend lifespan. PNAS; 106: 3502 - 3506, Mar 2009. (WB) Welzel AT, et al. 2014. Biochemistry 24:3908. (IP)
Product Citations: Chiang ACA, et al. 2018. Am J Pathol. 188:739. PubMed
RRID: AB_2715855 (BioLegend Cat. No. 806005) AB_2564692 (BioLegend Cat. No. 806001)
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family: APP/β-Amyloid
Gene ID: 351
UniProt: View information about APP 573-596 on UniProt.org
Clone: 1G6
Regulatory Status: RUO
Other Names: AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously: Signet Catalog# 9180-02Signet Catalog# 9180-05 and 9180-10Covance Catalog# SIG-39180
Isotype: Mouse IgG2b, κ