Biolegend, 815601, Purified anti-Histone H3 Trimethyl (Lys9) Antibody (Previously Covance catalog# MMS-5079), 100microl

Histone H3 is a 17 kD nuclear protein that is a component of an octamer containing pairs of each of four core histones (H2A, H2B, H3, H4). The core histones create nucleosome structure of chromosomal fiber in eukaryotes and are dynamic in gene regulation. The histones exhibit cell cycle-dependent transcriptional regulation except for the variant H3.3 which is cell cycle-independent. Histone H3 can be modified by phosphorylation, acetylation, ubiquitination, ribosylation, and methylation. Histone H3 has been shown to interact with Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1.
100microl
Verified Reactivity: Human, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This monoclonal antibody was raised against a synthetic peptide from Human Histone H3, tri-methylated at Lysine-9.
Formulation: Phosphate-buffered solution.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/ml
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: WB, IHC-F
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. The optimal working dilution should be determined for each specific assay condition. • WB: 1:100 - 1:1000* • IHC: 1:50 - 1:100 Tissue: Frozen sections Pretreatment: None Primary Incubation: 24 hours at 4°C
Application Notes: This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC). * Expected MW: 17 kD WB Positive Control: HeLa cell lysate IHC Positive Control: Rat Brain This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
Product Citations: Manou M, et al. 2023. Cells. :12. PubMed
RRID: AB_2564794 (BioLegend Cat. No. 815601)
Biology Area: Cell Biology, Neuroscience, Signal Transduction
Gene ID: 8350
UniProt: View information about Histone H3 on UniProt.org
Clone: 6F12-H4
Regulatory Status: RUO
Other Names: Histone H3.1, histone H3/a, histone 1, H3/a, H3 histone family, member A
Previously: Covance Catalog# MMS-5079
Isotype: Mouse IgG1