Biolegend, 816609, Alexa Fluor® 647 anti-Tau, 1-100 Antibody, 25μg

Tau protein promotes microtubule assembly and stability. Tau is abundant in neurons of the central nervous system, and is expressed at low levels in astrocytes and oligodendrocytes. Abnormal hyper-phosphorylation, aggregation, and toxic gain of function of tau is associated with several neurological disorders, including Alzheimer’s disease (AD). The major building block of neurofibrillary lesions in AD brains consists of paired helical filaments (PHFs) of abnormally hyperphosphorylated tau. Six isoforms of tau are generated by alternative splicing of the MAPT gene. These isoforms are distinguished by the number of tubulin binding domains, 3 (3R) or 4 (4R), in the C-terminal of the protein and by one (1N), two (2N), or no (0N) inserts in the N-terminal domain. Tau isoforms are differentially expressed during development.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This monoclonal antibody was raised against full-length human tau protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-P -Quality tested IHC, WB - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1 – 5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: This antibody is reactive to amino acid residues 1-100 of human Tau. The epitope lies within amino acids 6-18. This clone is specific for all six isoforms of human tau protein. This antibody clone has been reported for use on IHC of 4% PFA-fixed free floating sections5. This antibody is exclusively provided by BioLegend.
RRID: AB_2721436 (BioLegend Cat. No. 816609)
Structure: Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.
Distribution: Tissue distribution: Central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle. Cellular distribution: cytoskeleton, nucleus, plasma membrane and cytosol.
Function: Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Interaction: Tau interacts with Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine/threonine-protein kinase Sgk1, Laforin, Alpha-synuclein
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family: Tau
Antigen References: Augustinack JC, et al. 2002. Acta Neuropath. 103(1):26-35. Seubert P, et al. 1995. J Biol Chem. 270(32):18917-22. Dong Y, et al. 2012. PLoS One. 7(6):e39386. Simic G, et al. 2016. Biomolecules. 6(1):6. Dai CL, et al. 2017. Alzheimers Res Ther. 9(1):1. (IHC, WB) [PubMed] Baazaoui N, et al. 2017. Alzheimers Res Ther. 9:45. ( WB) [PubMed] Dai CL, et al. 2015. J neural Transm (Vienna). 122:607.
Gene ID: 4137
UniProt: View information about Tau 1-100 on UniProt.org
Clone: 43D
Regulatory Status: RUO
Other Names: Microtubule-associated protein tau, PHF-tau, paired helical filament-tau, neurofibrillary tangle protein, microtubule-associated protein tau, isoform 4, G protein beta1/gamma2 subunit-interacting factor 1
Isotype: Mouse IgG1, κ