Biolegend, 824803, Purified anti-TREM-2 Antibody, 25μg

TREM-2 is a 26 kD transmembrane glycoprotein that consists of a single extracellular immunoglobulin-like domain, a transmembrane region with a charged lysine residue and a short cytoplasmic tail. It associates with DNAX-activation protein 12 (DAP12) for signaling and function. TREM-2 is expressed on immature monocyte-derived dendritic cells. After activation by microbial products or tumor necrosis factor (TNF) and TNF-related proteins, dendritic cells downregulate the expression of TREM-2. TREM-2 is also expressed by osteoclasts and microglia, where it is involved in bone modeling and brain function, respectively. Another role of TREM-2 might be promoting the removal of apoptotic cells, organic matrix and macromolecules by microglia.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse TREM-2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Koga T, et al. 3004. Nature. 428:758. (FC, IP)
RRID: AB_2922646 (BioLegend Cat. No. 824803) AB_2922646 (BioLegend Cat. No. 824804)
Structure: 26 kD transmembrane glycoprotein, member of the immunoglobulin superfamily
Distribution: Subset of macrophages, osteoclasts and microglia, immature dendritic cells
Function: Removal of apoptotic cells, organic matrix and macromolecules, bone modeling
Interaction: DNAX-activation protein 12 (DAP12)
Biology Area: Cell Biology, Neuroinflammation, Neuroscience
Antigen References: Leyns CEG, et al. 2019. Nat Neurosci. 22:1217-22. Zhong L, et al. 2019. Nat Commun. 10:1365. Parhizkar S, et al. 2019. Nat Neurosci. 22:191-204. Filipello F, et al. 2018. Immunity. 48:979-91.
Gene ID: 83433
UniProt: View information about TREM2 on UniProt.org
Clone: 6E9
Regulatory Status: RUO
Other Names: Triggering receptor expressed on myeloid cells 2, Trem, TREM-2, Trem2a, Trem2b, Trem2c, TREM2
Isotype: Rat IgG2b, κ