Biolegend, 825801, Purified anti-HSF1 Antibody (Previously Covance catalog# MRT-5113), 100microl

HSF1 is a DNA-binding protein that exists as an inactive monomer in a complex with Hsp40/Hsp70 and Hsp90.  Upon stress, HSF1 is released from the chaperone complex and trimerizes.  HSF1 is then transported into the nucleus where it is hyperphosphorylated and binds to DNA containing heat shock elements (HSE) and activates transcription of proteins such as HSP70.  HSP70 is known to inhibit the tau and alpha synuclein aggregation seen in Alzheimer’s Disease and Parkinson’s Disease.
100microl
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: This monoclonal antibody was raised against mouse recombinant HSF-1 protein and was mapped to amino acids 425-439.
Formulation: Phosphate-buffered solution.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/mL
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: IHC-P - Quality tested WB, ICC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Mathew A, et al. 2001. Mol. Cell. Biol. 21:7163. (WB, ICC)
RRID: AB_2564892 (BioLegend Cat. No. 825801)
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Gene ID: 3297
UniProt: View information about HSF1 on UniProt.org
Clone: 4B4
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: Heat shock factor protein 1, heat shock transcription factor 1, HSF 1, HSTF 1
Previously: Covance Catalog# MRT-5113
Isotype: Rat IgG2a, κ