Biolegend, 828001, Purified anti-Histone H4 Monomethyl (Lys20) Antibody (Previously Covance catalog# MMS-5286), 100microl

Histone proteins are classified into core histones (H2A, H2B, H3, H4) and linker histones (H1, H5). Core histones form an octamer, which contains two H2A-H2B dimers and one H3-H4 tetramer. Core histones are predominantly globular except for the unstructured N-terminal tails. Posttranslational modifications, such as acetylation, methylation, phosphorylation, ubiquitination, SUMOylation and ADP-ribosylation occur in histone tails. Histone modifications induce changes of chromatin structure and thereby affect the accessibility of transcription factors, nuclear proteins and enzymes to genomic DNA, resulting in gene activation or repression. It is known that histone modifications play critical roles in DNA repair, DNA replication, transcription regulation, alternative splicing and chromosome condensation and some diseases including autoimmune diseases and cancers.
100microl
Verified Reactivity: Human, Rat, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This monoclonal antibody was raised against a synthetic peptide conjugated to KLH containing methylated lysine 20 of human Histone H4.
Formulation: Phosphate-buffered solution.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: IHC-P - Quality tested WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 0.1 - 1 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Shirakata Y, et al. 2014. J. Reprod. Dev. 60:383. (IHC-P)
RRID: AB_2564916 (BioLegend Cat. No. 828001)
Structure: Histone H4 is a 103 amino acid protein with an apparent molecular mass of 12 kD
Cell Type: Mesenchymal Stem Cells
Biology Area: Cell Biology, Neuroscience, Stem Cells
Gene ID: 8359
UniProt: View information about Histone H4 on UniProt.org
Clone: 5E10-D8
Regulatory Status: RUO
Other Names: histone H4, histone 1, H4a, H4 histone family member A
Previously: Covance Catalog# MMS-5286
Isotype: Mouse IgG1, κ