Biolegend, 835403, Purified anti-Neurofilament H & M (NF-H/NF-M), Non-Phosphorylated Antibody, 25μg

Neurofilaments (NF) are approximately 10 nanometer intermediate filaments found in neurons. They are a major component of the neuronal cytoskeleton, and function primarily to provide structural support for the axon and to regulate the axon diameter. There are three major NF subunits, and the names given to these subunits are based upon the apparent molecular mass of the mammalian subunits on SDS-PAGE. The light or lowest NF (NF-L) runs at 68-70 kD. The medium or middle NF (NF-M) runs at about 145-160 kD, and the heavy or highest NF (NF-H) runs at 200-220 kD. However, the actual molecular weight of these proteins is considerably lower due to the highly charged C-terminal regions of the molecules. The level of NF gene expression correlates with the axonal diameter, which controls how fast electrical signals travel down the axon. Mutant mice with NF abnormalities have phenotypes resembling amyotrophic lateral sclerosis. NF immunostaining is common in diagnostic neuropathology. It is useful for differentiating neurons (positive for NF) from the glia (negative for NF).
25μg
Verified Reactivity: Rat, Mouse, Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality testedWB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Immunohistochemistry. For Immunohistochemistry of paraffin-embedded tissues, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Cell bodies and dendrites are generally unstained. Other cells and tissues are unreactive except for peripheral axons.
Application References(PubMed link indicates BioLegend citation): Wang J, et al. 2001. FEBS Lett. 19;507(1): 81-7. (WB)
Product Citations: Zhu PP, et al. 2022. Hum Mol Genet. 31:2779. PubMed
RRID: AB_2716096 (BioLegend Cat. No. 835403) AB_2716099 (BioLegend Cat. No. 835404)
Structure: The medium or middle NF (NF-M) runs at about 145-160 kD, and the heavy or highest NF (NF-H) runs at 200-220 kD.
Distribution: Tissue Distribution: CNS, peripheral nerves and glandular cells of the prostate Cellular distribution: cytoskeleton, nucleus, cytosol, and mitochondrion
Function: Neurofilaments are the major components of the neuronal cytoskeleton. They provide axonal support and regulate axon diameter.
Cell Type: Mature Neurons
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family: Intermediate Filaments, Phospho-Proteins
Gene ID: 4744
UniProt: View information about Neurofilament HM NF-H NF-M on UniProt.org
Clone: SMI 33
Regulatory Status: RUO
Other Names: Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, neurofilament triplet H protein
Isotype: Mouse IgM, κ