Biolegend, 837904, Purified anti-Neurofilament Marker (pan axonal, cocktail) Antibody, 100μg

Neurofilaments (NF) are approximately 10 nanometer intermediate filaments found in neurons. They are a major component of the neuronal cytoskeleton and their function is primarily to provide structural support for the axon and to regulate axon diameter. There are three major NF subunits, and the names given to these subunits are based upon the apparent molecular mass of the mammalian subunits on SDS-PAGE. The light or lowest (NF-L) runs at 68-70 kD, the medium or middle (NF-M) runs at about 145-160 kD, and the heavy or highest (NF-H) runs at 200-220 kD. However, the actual molecular weight of these proteins is considerably lower due to the highly charged C-terminal regions of the molecules. The level of NF gene expression correlates with the axonal diameter, which controls how fast electrical signals travel down the axon. Mutant mice with NF abnormalities have phenotypes resembling amyotrophic lateral sclerosis. NF immunostaining is common in diagnostic neuropathology. It is useful for differentiating neurons (positive for NF) from glia (negative for NF).
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Homogenized hypothalami recovered from Fischer 344 rats.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested WB - Verified EM, ICC, IHC-F - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/mL is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunocytochemistry1, 6, 12, 16, 19, immunofluorescent staining3, 8, 9, 18. SMI 312 is a mixture of monoclonal antibodies that react against complex networks of axons. It is directed against extensively phosphorylated axonal epitopes on neurofilaments M and H. SMI 312 has been selected to provide a specific marker for axons in tissue sections and cultures. In contrast to individual anti-phosphoneurofilament antibodies that identify different subsets of neurofilament phosphoepitopes, which are suitable for defining functional and regional differences in normal and pathologic axons, SMI 312 is a convenient marker for axons in general. SMI 312 visualizes axons in an area-specific maturation pattern in human fetal brain. The antibody cocktail defines nuclear borderlines and is useful in establishing early connectivity with SMI 311, anti-neurofilament (not phosphorylated) identified dendrites. SMI 312 visualizes aberrantly sprouting axons in neuritic plaques derived from cortico-cortical fibers in Alzheimer's disease and identifies loss of synaptic circuitry proposed to be the basis of memory.
Application References(PubMed link indicates BioLegend citation): Sternberger LA, et al. 1982. Proc. Natl. Acad. Sci. USA. 79:1326. (IHC, ICC) Choi Y, et al. 2008. Genes & Dev. 22:2485. (IHC) PubMed BussiFre T, et al. 2004. Am. J. Pathol. 165:987. (IHC-F) Chung RS, et al. 2003. J. Neurosci. 23:3336. (IHC) De Repentigny Y, et al. 2011. PLoS One. 6:e21093. (IHC) Wilkins A, et al. 2003. J. Neurosci. 23:4967. (ICC) Rudinskiy N, et al. 2012. Nat. Neurosci. 15:1422. (IHC) Wang JY, et al. 2014. Dev. Cell. 28:670. (EM) Canetta SE, et al. 2011. PLoS One. 6:e25108. (ICC) PubMed Nicaise C, et al. 2012. J. Neurotrauma. 29:2748. (IHC) Ma M, et al. 2013. Neurobiol. Dis. 56:34. (IHC) Zurashvili T, et al. 2013. Mol. Cell Biol. 33:1027. (ICC) Powers BE, et al. 2013. Proc. Natl. Acad. Sci. USA. 110:4075. (IHC) Riddle A, et al. 2012. Stroke. 43:178. (IHC) Sahni V, et al. 2010. Neurosci. 30:1839. (IHC) Liu HY, et al. 2013. J. Neurosci. 33:11479. (ICC) Nicaise C, et al. 2013. J. Neurotrauma. 30:1092. (IHC) Klusch A, et al. 2013. J. Invest. Dermatol. 133:1387. (ICC) PubMed Huang TN, et al. 2014. Nat. Neurosci. 17:240. (ICC)
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RRID: AB_2566782 (BioLegend Cat. No. 837904)
Structure: Three major neurofilament subunits. Its names given to these subunits are based upon the apparent molecular mass of the mammalian subunits on SDS-PAGE: The medium or middle (NF-M) runs at about 145-160 kD and the heavy or highest (NF-H) runs at 200-220 kD.
Distribution: Tissue distribution: CNS, peripheral nerves, and glandular cells of the prostate. Cellular distribution: Cytoskeleton, nucleus, cytosol, and mitochondrion.
Function: Neurofilaments are the major components of the neuronal cytoskeleton. They provide axonal support and regulate axon diameter.
Cell Type: Mature Neurons
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family: Intermediate Filaments
Antigen References: 1. Petzold A. 2005. J. Neurol. Sci. 233:183. PubMed
Gene ID: 474747444741
UniProt: View information about Neurofilament Marker on UniProt.org
Clone: SMI 312
Regulatory Status: RUO
Other Names: SMI-312, SMI312
Isotype: Mouse IgG1 κ/ IgG3 κ