Biolegend, 845102, Purified anti-Syntrophin Antibody, 100μg

Syntrophins are a family of five 60 kD adapter proteins that use their multiple protein interaction domains (two PHDs and a PDZ) to localize various signaling proteins (kinases, ion/water channels, or NOS) to specific intracellular locations. In neurons, syntrophins are known as the postsynaptic density protein (PSD) that serves to connect neurotransmitter receptors (such as nAChRs) to the cytoskeleton.
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Whole purified syntrophin from Torpedo californica electric organ postsynaptic membrane.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICFC, ICC, IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The epitope has been mapped to amino acids 108-120 within the a-syntrophin PDZ domain.
Application References(PubMed link indicates BioLegend citation): Froehner S, et al. 1987. J. Cell Biol. 104:1633. (WB, IHC) Gee S, et al. 1998. J. Neurosci. 18:128. (IP) Ort T, et al. 2001. EMBO 20:4013. (IP, WB) Adams M, et al. 2001. J. Cell Biol. 155:113. (IF) Williams M, et al. 1999. J. Cell Biol. 144:1259. (IHC)
Product Citations: Martin CA, et al. 2022. Regen Biomater. 9:rbac031. PubMed
RRID: AB_2566133 (BioLegend Cat. No. 845102)
Structure: Monomer and homodimer. Syntrophins consist of a conserved domain, two pleckstin homology domains, a PDZ domain, and a highly conserved C-terminal syntrophin unique (SU) domain. Expected MW: 58 kD.
Function: Adapter protein that binds to and probably organizes the subcellular localization of a variety of membrane proteins. May link various receptors to the actin cytoskeleton and the dystrophin glycoprotein complex.
Interaction: Interacts with the other members of the syntrophin family SNTB1 and SNTB2; SGCG and SGCA of the dystrophin glycoprotein complex; NOS1; GRB2; the sodium channel proteins SCN4A and SCN5A; F-actin and calmodulin; Interacts with MYOC.
Biology Area: Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family: Postsynaptic proteins
Gene ID: 6640
UniProt: View information about Syntrophin on UniProt.org
Clone: 1351
Regulatory Status: RUO
Other Names: Dystrophin-associated protein A1 acidic component 1, Pro-TGF-alpha cytoplasmic domain-interacting protein 1, TACIP1, Syntrophin-1, SNT1, LQT12,dJ1187J4.5
Isotype: Mouse IgG1, κ