Biolegend, 845302, Purified anti-Notch1β Antibody, 100μg

Notch1 signaling is involved in tumorigenesis as well as in determining the fates of neural and non-neural cells during development and in adults. Alterations in the proteolysis of Notch1 by gamma-secretase could be involved in the pathogenesis of Alzheimer’s Disease.
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KLH conjugated peptide corresponding to aa 1721-1745 of human Notch1 (VQSETVEPPPPAQLHFMYVAAAAFV)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested Direct ELISA - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemcical staining. For immunohistochemistry, a concentration range of 1 - 10 µg/ml is recommended. For Direct ELISA, the suggested use of this reagent is 0.05 - 0.1 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone 6B11/Notch has been tested by Direct ELISA on Notch ß (Nß) peptide coated plates. It has not been evaluated in a sandwich ELISA format.
RRID: AB_2616642 (BioLegend Cat. No. 845302)
Structure: Notch1 is a 272 kD protein.
Distribution: Highly expressed in the brain, lung, and thymus (CD4-CD8- cells and CD4-CD8+ cells). Lower levels of expression in spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney and heart is observed.
Function: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Affects the implementation of differentiation, proliferation and apoptotic programs.
Interaction: Notch1 is processed in a manner similar to APP, generating Nβ peptides (S4 cleavage) and the Notch intracellular domain (NICD, S3 cleavage) which translocates to the nucleus and acts as an essential regulator of transcription factors.
Ligand/Receptor: Receptor for the membrane ligands Jagged1, Jagged2, and Delta1 to regulate cell fate decisions.
Cell Type: Thymocytes
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Synaptic Biology
Molecular Family: Postsynaptic proteins
Antigen References: 1. Okochi M, et al. 2006. J. Biol. Chem. 281(12):7890. 2. Wanngren J, et al. 2012. J. Biol. Chem. 287(39):32640. 3. Tanveer R, et al. 2012. J. Biol. Chem. 287(41):34709.
Gene ID: 4851
UniProt: View information about Notch1beta on UniProt.org
Clone: 6B11/Notch
Regulatory Status: RUO
Other Names: TAN1, Translocation-Associated Notch Protein TAN-1, Neurogenic Locus Notch Homolog Protein 1, hN1
Isotype: Mouse IgG2b, κ