Biolegend, 847405, Alexa Fluor® 594 anti-ATG5 Antibody, 25μg

Autophagy protein 5 (ATG5) is involved in autophagic vesicle formation. ATG5 forms a complex with autophagy protein 12 (ATG12), which has E3 ubiquitin ligase activity, and is associated with autophagosome vesicle formation. ATG5 detaches from membranes immediately before or after autophagosome formation. ATG5 is also involved in mitochondrial quality control after oxidative damage, the innate anti-viral immune response, and apoptosis.
25μg
Verified Reactivity: Human, Mouse, Rat, Drosophila
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: ATG5 peptide conjugated to KLH.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Application Notes: Reactivity to Drosophila was only verified with the purified format.
RRID: AB_2750295 (BioLegend Cat. No. 847405) AB_2750296 (BioLegend Cat. No. 847406)
Structure: Human ATG5 is a 275 amino acid protein with a molecular mass of 32 kD.
Distribution: Tissue distribution: Ubiquitous. Cellular distribution: Vacuole, lysosome, and to a lesser extent mitochondria.
Function: ATG5 is involved in many cellular processes including autophagic vesicle formation, mitochondrial quality control after oxidative damage, innate anti-viral immune response, and apoptosis.
Interaction: ATG12, LC3B, ATG16, TECPR1, Autophagy-linked FYVE protein.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
Molecular Family: Autophagosome Markers
Antigen References: 1. Uchiyama Y, et al. 2008. Histochem. Cell Biol. 129:407. PubMed 2. Wu H, et al. 2014. Int. J. Biol. Sci. 10:1072. PubMed 3. Subramani S, Malhotra V. 2013. EMBO Rep. 14(2):143. PubMed
Gene ID: 9474
UniProt: View information about ATG5 on UniProt.org
Clone: 177.19
Regulatory Status: RUO
Other Names: Autophagy protein 5
Isotype: Mouse IgG1, κ