Biolegend, 849401, Purified anti-β-Dystroglycan Antibody, 25μg

Dystroglycan is a central component of dystrophin-glycoprotein complex that is encoded by the DAG1 gene. Dystroglycan links the cytoskeletal structure to the extracellular matrix. Dystroglycan is proteolytically cleaved to 2 functional peptides, α- and β-dystroglycan. β-dystroglycan is a transmembrane protein, which acts as a cell adhesion receptor in human tissues. The interactions between β-dystroglycan and dystrophin and utrophin scaffolds axin to the cytoskeleton. β-dystroglycan has also been reported to play a role in cell adhesion-mediated signaling and is implicated in cell polarity.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KLH-conjugated peptide corresponding to the last 15 amino acids (KNMTPYRSPPPYVPP) of L-Dystroglycan.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Sumita K, et al. 2007. J. Neurochem. 100(1):154. (IP, WB)
RRID: AB_2687292 (BioLegend Cat. No. 849401) AB_2687293 (BioLegend Cat. No. 849402)
Structure: Dystroglycan is an 895 amino acid protein with a molecular mass of ~97 kD. β-dystroglycan, a cleavage product of dystroglycan, is a 242 amino acid protein with a molecular mass of ~43 kD.
Distribution: Tissue distribution: Primarily expressed in muscle tissue. Also present in other tissue including CNS. Cellular distribution: nucleus, plasma membrane, and cytoskeleton.
Function: β-dystroglycan is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization.
Interaction: Interacts with dystrophin, utrophin and other cytoskeletal components such as axin.
Ligand/Receptor: Receptor for dystrophin and utrophin.
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family: Adhesion Molecules
Antigen References: 1. Murphy S and Ohlendieck K. 2015. Comput. Struct. Biotechnol. J. Nov 26;14:20-7. 2. Moore CJ and Winder SJ. 2012. Neuromuscul. Disord. Nov: 22(11): 959.
Gene ID: 1605
UniProt: View information about beta-Dystroglycan on UniProt.org
Clone: 4F7
Regulatory Status: RUO
Other Names: Beta-dystroglycan, Beta-DG, DAG1
Isotype: Mouse IgG2b, κ