Biolegend, 849802, Purified anti-Flotillin-1 Antibody, 100μg

The flotillin family is composed of two highly homologous proteins, flotillin-1 and flotillin-2 that share ~50% amino acid sequence similarity. Flotillins are ubiquitously expressed, membrane-associated proteins, and were shown to be associated with lipid microdomains called membrane rafts. Flotillins are present in various cellular compartments, including the plasma membrane and endosomes, and they have been suggested to function in cellular signaling and membrane trafficking processes, including endocytosis and endosomal trafficking. 100μg Verified Reactivity: Human, Rat, Mouse Antibody Type: Monoclonal Host Species: Rat Immunogen: Human Flotillin-1 recombinant protein corresponding to amino acids 1-270 expressed in E. coli. Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Preparation: The antibody was purified by affinity chromatography. Concentration: 0.5 mg/ml Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Application: WB - Quality tested ICC - Verified Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. For immunocytochemistry microscopy, a concentration range of 1.0-5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. Application Notes: This antibody specifically recognizes Flotillin-1 but not Flotillin-2, verified using WB. Product Citations: Buenaventura T, et al. 2019. PLoS Biol. 17:e3000097. PubMed RRID: AB_2687308 (BioLegend Cat. No. 849801) AB_2687309 (BioLegend Cat. No. 849802) Structure: Flotillin-1 is a 427 amino acid protein with a molecular mass of 47 kD. Distribution: Tissue distribution: Flotillin-1 is ubiquitously expressed. Cellular distribution: Flotillin-1 is mainly expressed in the cytosol and endosomes. Function: Flotillins are thought to interact with the cytoskeleton and function in a number of cellular processes such as endocytosis. Interaction: Flotillin-1 interacts with cbl-associated protein/ponsin (CAP). Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Misfolding and Aggregation, Protein Trafficking and Clearance Antigen References: 1. Otto G et al., 2011. Journal of Cell Science. 124: 3933. Gene ID: 10211 UniProt: View information about Flotillin-1 on UniProt.org Clone: W16108A Regulatory Status: RUO Other Names: Flotillin 1, Flot-1, Integral Membrane Component of Caveolae Isotype: Rat IgG2a, κ