Biolegend, 849803, HRP anti-Flotillin-1 Antibody, 25μg

The flotillin family is composed of two highly homologous proteins, flotillin-1 and flotillin-2 that share ~50% amino acid sequence similarity. Flotillins are ubiquitously expressed, membrane-associated proteins, and were shown to be associated with lipid microdomains called membrane rafts. Flotillins are present in various cellular compartments, including the plasma membrane and endosomes, and they have been suggested to function in cellular signaling and membrane trafficking processes, including endocytosis and endosomal trafficking.
25μg
Verified Reactivity: Human, Rat, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human Flotillin-1 recombinant protein corresponding to amino acids 1-270 expressed in E. coli.
Formulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone specifically recognizes Flotillin-1 but not Flotillin-2, verified using WB.
RRID: AB_2728577 (BioLegend Cat. No. 849803)
Structure: Flotillin-1 is a 427 amino acid protein with a molecular mass of 47 kD.
Distribution: Tissue distribution: Flotillin-1 is ubiquitously expressed. Cellular distribution: Flotillin-1 is mainly expressed in the cytosol and endosomes.
Function: Flotillins are thought to interact with the cytoskeleton and function in a number of cellular processes such as endocytosis.
Interaction: Flotillin-1 interacts with cbl-associated protein/ponsin (CAP).
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Misfolding and Aggregation, Protein Trafficking and Clearance
Antigen References: 1. Otto G et al., 2011. Journal of Cell Science. 124: 3933.
Gene ID: 10211
UniProt: View information about Flotillin-1 on UniProt.org
Clone: W16108A
Regulatory Status: RUO
Other Names: Flotillin 1, Flot-1, Integral Membrane Component of Caveolae
Isotype: Rat IgG2a, κ