Biolegend, 850002, Purified anti-Connexin 43, 247-382 Antibody, 100μg

Connexins are a family of transmembrane proteins that assemble to form vertebrate gap junctions, thus also called Gap Junction Proteins. So far, 21 Connexins have been discovered in humans. The gene GJA1 encodes Connexin 43 (Cx43), which is the most expressed Connexin, and can be found in many human tissues including skin, bone, and brain. Cx43 has been reported to be involved in synchronized contraction of the heart as well as embryonic development. Besides its functions as a channel protein, Cx43 also performs channel-independent functions. During brain and heart development, cytoplasmic Cx43 regulates the microtubule network and thus affects cell migration and polarity. In addition, mitochondrial GJA1 has also been reported to promote cell survival by down-regulating the intrinsic apoptotic pathway during conditions such as oxidative stress.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KLH-conjugated peptide corresponding to amino acids 247-382 of Connexin 43
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1 to 5 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2721730 (BioLegend Cat. No. 850001) AB_2721731 (BioLegend Cat. No. 850002)
Structure: Connexin 43 (GJA1) is a 382 amino acid protein with a molecular mass of 43 kD.
Distribution: Tissue distribution: expressed in most tissues, enriched in brain, endocrine, muscle, bone marrow, immune System, ovaries, testes, and Skin. Cellular distribution: plasma membrane, cytosol, mitochondria, golgi apparatus, cytoskeleton and endoplasmic reticulum membrane.
Function: Connexin 43 plays a role in cell-to-cell communication by forming channels or gap junctions between cells. It allows for the transport of nutrients, charged particles (ions), and other small molecules that carry necessary communication signals between cells.
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers
Antigen References: Grek CL, et al. 2016. Cancer Lett. 374(1):117 Lilly E, et al. 2016. Semin Cell Dev Biol. 50:4 Michela P, et al. 2015. Eur J Pharmacol. 768:71
Gene ID: 2697
UniProt: View information about Connexin 43 247-382 on UniProt.org
Clone: 2F5
Regulatory Status: RUO
Other Names: GJA1, Cx43, Gap Junction Protein Alpha 1
Isotype: Mouse IgG1, κ