Biolegend, 850401, Purified anti-Rab7A Antibody, 25μg

Rab7, a small GTPase of the Rab family, plays multiple roles including vesicular transport, endocytic trafficking, and autophagy. Rab7 is associated with both the endosome and lysosome, and it facilitates endosomal maturation, transport from the late endosome to the lysosome, and positioning of the endosome and lysosome via regulating their movement along cytoskeleton. Consistently, mutations or dysfunctions of Rab7 result in traffic disorders, which cause various diseases, such as neuropathy, cancer and lipid metabolism disease. Rab7 also plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses.
25μg
Verified Reactivity: Human, Rat, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Full-length recombinant human Rab7A protein expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested Direct ELISA, ICC, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 µg per ml. For immunocytochemistry, the suggested usage is 1.0 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2715874 (BioLegend Cat. No. 850401) AB_2715875 (BioLegend Cat. No. 850402)
Structure: Rab7A is a 207 amino acid protein with a molecular mass of 23 kD.
Distribution: Tissue distribution: Rab7A is ubiquitously expressed. Cellular distribution: Plasma membrane, cytosol, lysosome, endosome, and Golgi apparatus.
Function: Rab7A is involved in the endocytic pathway and autophagy.
Interaction: Rab7A interacts with RILP, Rab1A, Rab11A, and UBC.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
Molecular Family: Autophagosome Markers
Antigen References: 1. Guerra F, Bucci C. 2016. Cells. 5(3).
Gene ID: 7879
UniProt: View information about Rab7A on UniProt.org
Clone: W16034A
Regulatory Status: RUO
Other Names: Rab7, Ras-Related Protein Rab-7a, Ras-Associated Protein RAB7
Isotype: Rat IgG2a, κ