Biolegend, 850801, Purified anti-Adenomatous polyposis coli (APC) Antibody, 25μg

The APC gene provides instructions for making the APC protein, which plays a critical role in several cellular processes. The APC protein acts as a tumor suppressor, which means that it keeps cells from growing and dividing too fast or in an uncontrolled way. It helps control how often a cell divides, how it attaches to other cells within a tissue, and whether a cell moves within or away from a tissue. This protein also helps ensure that the number of chromosomes in a cell is correct following cell division. The APC protein accomplishes these tasks mainly through association with other proteins, especially those that are involved in cell attachment and signaling. One protein with which APC associates is beta-catenin. Beta-catenin helps control the activity (expression) of particular genes and promotes the growth and division (proliferation) of cells and the process by which cells mature to carry out specific functions (differentiation). Beta-catenin also helps cells attach to one another and is important for tissue formation. Association of APC with beta-catenin signals for beta-catenin to be broken down when it is no longer needed.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: N-terminal APC-MBP fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence microscopy1.
Application References(PubMed link indicates BioLegend citation): Boehlke C, et al. 2013. PLoS One. 8(5):e62165. (IF) Hadjihannas MV, et al. 2012. EMBO Rep. 13(4):347-54. (WB) Tanneberger K, et al. 2011. J. Biol. Chem. 286(22):19204-14. (WB)
RRID: AB_2716017 (BioLegend Cat. No. 850801) AB_2716018 (BioLegend Cat. No. 850802)
Structure: APC is a 2843 amino acid protein with a molecular weight of 311 kD.
Distribution: Tissue distribution: expressed in wide variety of normal tissues. Cellular distribution: cellular junctions, cytoplasm and membranous expression.
Function: APC is a tumor suppressor involved in Wnt signaling. It is also involved in additional processes including cell migration and adhesion, transcriptional activation and apoptosis.
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family: Tumor Suppressors
Antigen References: 1. Barth AI, et al. 2008. Semin. Cell. Dev. Biol. 19(3):245-51.
Gene ID: 324
UniProt: View information about Adenomatous polyposis coli on UniProt.org
Clone: Ali 12-28
Regulatory Status: RUO
Other Names: APC, BTPS2, DP2, DP2.5, DP3, PPP1R46, WNT signaling pathway regulator, adenomatosis polyposis coli tumor suppressor, deleted in polyposis 2.5, protein phosphatase 1, regulatory subunit 46, truncated adenomatosis polyposis coli
Isotype: Mouse IgG1, κ