Biolegend, 852701, Purified anti-Apo E, 109-116 Antibody, 25μg

Apolipoprotein E (Apo E) belongs to a class of apolipoproteins that mediate the binding, internalization, and catabolism of lipoprotein particles. Apo E is produced by the liver and macrophages in peripheral tissues where it regulates cholesterol metabolism. In the central nervous system, Apo E is primarily produced by astrocytes and plays an important role in transporting cholesterol to neurons via Apo E receptors. Apo E has three alleles (ApoE2, ApoE3 and ApoE4) that differ from each other by only one or two amino acids at positions 112 and 158. ApoE 4 is one of the largest known genetic risk factors for late-onset Alzheimer's disease. Furthermore, Apo E is associated with cardiovascular disease, Lipoprotein Glomerulopathy and Sea-Blue Histiocyte Disease.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedDirect ELISA - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. For Direct ELISA applications, a concentration range of 0.004 - 0.4 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Mahley RW. 2016. Arterioscler Thromb Vasc Biol. 36: 1305-1315. Riedel BC, et al. 2016. J Steroid Biochem Mol Biol. 160:134-47. Tai LM, et al. 2016. Acta Neuropathol. 131(5):709-23. Kim J, et al. 2014. Mol. Cells. 37:767 Holtzman DM, et al. 2012. Cold Spring Harb. Perspect. Med. 2:a006312
RRID: AB_2728597 (BioLegend Cat. No. 852701) AB_2728598 (BioLegend Cat. No. 852702)
Structure: Human APO E is a 299 amino acid protein with a molecular mass of 32 kD, and exists as 3 major isoforms, APO E2, APO E3, and APO E4.
Distribution: Tissue sources: Ubiquitously expressed. Highest levels in liver, brain, spleen, adrenal gland, and kidney. Cellular distribution: Plasma membrane, nucleus, endoplasmic reticulum, golgi apparatus, endosome, and extracellular.
Function: Apo E is involved in lipoprotein metabolism as well as the transport of fat-soluble vitamins, and cholesterol. Apo E also forms complexes with amyloid beta peptides leading to its uptake and degradation by microglia.
Interaction: Tau, amyloid beta
Ligand/Receptor: LDL receptor, SORL1
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family: Apolipoproteins
Antigen References: Mahley RW. 2016. Arterioscler Thromb Vasc Biol. 36: 1305-1315. Riedel BC, et al. 2016. J Steroid Biochem Mol Biol. 160:134-47. Tai LM, et al. 2016. Acta Neuropathol. 131(5):709-23. Kim J, et al. 2014. Mol. Cells. 37:767 Holtzman DM, et al. 2012. Cold Spring Harb. Perspect. Med. 2:a006312
Gene ID: 348
UniProt: View information about Apo E 109-116 on UniProt.org
Clone: A17067B
Regulatory Status: RUO
Other Names: Apolipoprotein E, Alzheimer Disease 2, LDLCQ5, LPG, AD2
Isotype: Mouse IgG2b, κ