Biolegend, 856001, Purified anti-Dopamine Receptor D1 Antibody, 25μg

The D1 subtype of the dopamine receptor (DRD1) is a Gs/a-protein coupled receptor mainly expressed in the caudate putamen of the central nervous system. Upon binding to its ligand, DRD1 indirectly activates cyclic AMP-dependent protein kinase and regulates neuronal development. Two transcript variants, DRD1a and DRD1b exist in vivo. DRD1 has been discovered to modulate DRD2-mediated signaling pathway. DRD1 and DRD2 also form heterodimers via C-terminal regions, which has been reported to be involved in phospholipase C and CaMKII activation, and BDNF production.
25μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human DRD1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 10 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2783448 (BioLegend Cat. No. 856001) AB_2783449 (BioLegend Cat. No. 856002)
Structure: DRD1 is a 446 amino acid protein with an apparent molecular mass of ~ 49 kD.
Distribution: Tissue Distribution: Mainly expressed in the midbrain. Cellular Distribution: Plasma membrane.
Function: DRD1 is involved in dopamine related signaling and neuronal development.
Interaction: Interacts with DRD2, DNAJC14, and DRD1IP.
Ligand/Receptor: Receptor of Dopamine
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family: Adhesion Molecules, GPCR
Antigen References: Amare AT, et al. 2017. EPMA J. 8(3):211 Arango-Lievano M, et al. 2015. Med Sci (Paris). 31(5):546 Serretti A, et al. 2007. J Alzheimers Dis. 12(1):73
Gene ID: 181213488
UniProt: View information about Dopamine Receptor D1 on UniProt.org
Clone: A17050E
Regulatory Status: RUO
Other Names: DRD1, Dopamine Receptor D1
Isotype: Mouse IgG2b, κ