Biolegend, 856701, Purified anti-PAK1 Antibody, 25μg

PAK1 is a ubiquitously expressed protein that belongs to the group I PAK family of serine/threonine kinases, which are important mediators that link the Rho family GTPase signaling to cytoskeleton remodeling. PAK1 activation has been shown to play a role in tumorgenesis, actin dynamics, cell cycle progression, cell motility, and angiogenesis. The PAK family of kinases is affected in several brain diseases, including neurofibromatosis model of autism, Alzheimer’s Disease, Fragile X syndrome and schizophrenia 1 . Recently studies using electrophysiological recordings of hippocampal CA1 synaptic currents suggest that PAK1 plays a role in inhibitory synaptic transmission, where knocking out PAK affects the excitatory/inhibitory balance in the hippocampus, and may lead to dysregulation of synaptic strength.
25μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 0.2 - 2.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2783462 (BioLegend Cat. No. 856701)
Structure: PAK1 is a 545 amino acid protein with an apparent molecular mass of ~ 61 kD.
Distribution: Tissue distribution: Ubiquitously expressed in all tissues. Cellular distribution: Cytosol, plasma membrane, and nucleus.
Function: PAK1 plays an important role in regulating cytoskeletal dynamics, spine morphology, and synaptic plasticity.
Interaction: Forms homodimer, NISCH, DVL1, MUSK, CDC2L1, CDC2L2, p110C, CDC42/P21, RAC1, DSCAM, PDPK1, RAF1, NCK1, NCK2
Cell Type: Astrocytes, Microglia, Neurons, Oligodendrocytes
Biology Area: Neuroscience, Synaptic Biology
Molecular Family: Postsynaptic proteins
Antigen References: Xia S, et al. 2018. Small GTPases. 9(4):322 Kreis P, et al. 2009. Cell Signal. 21(3):384 Nikolic M. 2008. Mol Neurobiol. 37(2-3): 187 Salminen A, et al. 2008. Biochem Biophys Res Commun. 371(4):587
Gene ID: 50581847929431
UniProt: View information about PAK1 on UniProt.org
Clone: A17137A
Regulatory Status: RUO
Other Names: PAK1, p21-activated kinase 1
Isotype: Mouse IgG2a, κ