Biolegend, 862002, Purified anti-Pax6 Antibody, 100μg

Paired box protein Pax6 is a homeobox transcription factor belonging to a family of 9 proteins, which all share a 128 amino-acid DNA binding domain termed “paired domain” (PD). Homologs of Pax proteins exist in worms, flies, frogs, fish and birds. The Pax family has been shown to play important roles in organogenesis and development. The Pax family of proteins modify transcription activity and protein expression levels primarily through binding to the regulatory region of downstream genes. In addition to the PD domain, some Pax proteins, including Pax6, possess another DNA binding domain, a homeodomain (HD), which preferentially binds to sequence with “TAAT” core motif. Pax6 was first identified by its homology to the Drosophila Paired gene and is mainly expressed in the central nervous system, eye and pancreas. Mutations of Pax6 result in severe eye development abnormalities. Human heterozygous mutations of Pax6 result in aniridia, blindness, cataract and colobomas. In mice, homozygous Pax6 mutations lead to complete loss of ocular structure. In Drosophila , mutation of Pax6 homolog results in an eyeless phenotype. In addition to the eye development, pancreas abnormalities have been also observed in Pax6 deficiency mice.
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KLH conjugated peptide corresponding to amino acid sequence 174-188 of human Pax6.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICC - Quality tested IHC-P, IHC-F, WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. For formalin-fixed paraffin-embedded immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. For frozen tissue immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Reactivity is weaker in Rat compared to Human and Mouse.
Product Citations: Breitmeyer R, et al. 2023. Commun Biol. 6:472. PubMed Wang Y, et al. 2023. FASEB J. 37:e22776. PubMed Feng H, et al. 2021. Stem Cell Research. 53:102346. PubMed Cai T, et al. 2021. Cell Reports. 36(2):109337. PubMed Kano M, et al. 2019. Endocrinology. 160:1701. PubMed
RRID: AB_2801237 (BioLegend Cat. No. 862001) AB_2801238 (BioLegend Cat. No. 862002)
Structure: Human Pax6 is a 422 amino acid protein with a molecular mass of 46 kD.
Distribution: Tissue distribution: central nervous system, eye, and pancreas Cellular distribution: nucleus
Function: Pax6 is involved in the regulation of gene expression.
Interaction: Interacts with MAF and MAFB; interacts with TRIM11 Cellular targets: more than 200 target genes, including foxp2, pax6a, gata3, putf3 and more.
Cell Type: Neural Stem Cells, Neurons
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Synaptic Biology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: Chi N, et al. 2002. Trends Genet. 18(1): 41-7. Coutinho P, et al. 2011. Genome Res. 21(8):1349-59.
Gene ID: 50801850825509
UniProt: View information about Pax6 on UniProt.org
Clone: AD2.35
Regulatory Status: RUO
Other Names: Paired box protein Pax-6, Pax6, Pax, Aniridia type II protein, Oculorhombin
Isotype: Mouse IgG2a, κ