Biolegend, 865402, Purified anti-Rab8 Antibody, 100μg

Rab8 is a small GTP/GDP binding protein that belongs to the RAS superfamily. It is well established that Rab8 is an important participant of macroautophagy, and is essential for several apical transport pathways. Recruitment of Rab8a and TBK1 by the autophagy receptor optineurin initiates the autophagy process at specific protein aggregate site, which leads to the clearance of aggregated protein. Recent studies on C9ORF72, the gene that most commonly mutated in Amyotrophic Lateral Sclerosis and Frontotemporal Dementia (ALS-FTD), show that the C9ORF72/SMCR8/WDR41 complex acts as a GDP/GTP exchange factor for Rab8a, which facilitates the interaction between Rab8a and the autophagy receptor optineurin 4 .
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human Rab8A
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1 - 5 µg per ml. For immunocytochemistry, a concentration range of 1 - 5 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone cross-react with Rab8A and Rab8B.
RRID: AB_2810773 (BioLegend Cat. No. 865401) AB_2810774 (BioLegend Cat. No. 865402)
Structure: Rab8 is a 207 amino acid protein with an apparent molecular mass of ~24 kD.
Distribution: Tissue Distribution: ubiquitously expressed Cellular Distribution: Plasma membrane, cytoskeleton, cytosol, Golgi Apparatus, and endosome.
Function: Rab8 is a GTPase that plays an important role in intracellular membrane trafficking.
Interaction: MICALL1, MICALL2, Optineurin, MAP4K2
Cell Type: Astrocytes, Dopaminergic Neurons, GABAergic Neurons, Glutamatergic Neurons, Mature Neurons, Microglia, Neural Stem Cells, Neurons, Oligodendrocytes
Biology Area: Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Protein Trafficking and Clearance, Synaptic Biology
Molecular Family: Autophagosome Markers, Cytoskeletal Proteins, Organelle Markers, Tumor Suppressors
Antigen References: Zhang X and Gao N. 2016. Small GTPases. 7:59 Just WW and Peränen J. 2016. Biochim Biophys Acta. 1863:1006 Ao X, et al. 2014. Cell Death Differ. 21(3):348 Corbier C and Sellier C. 2017. Small GTPases. 8:181
Gene ID: 421817274117103
UniProt: View information about Rab8 on UniProt.org
Clone: A18073A
Regulatory Status: RUO
Other Names: Rab8, Rab8A
Isotype: Mouse IgG2b, κ