Biolegend, 865701, Purified anti-CHIP Antibody, 25μg

CHIP is an E3 ubiquitin-protein ligase known to ubiquitinate  misfolded chaperone-bound substrates and target them for proteasomal degradation. Hsp70, Hsc70, and Hsp90  are three chaperone proteins with which CHIP interacts. CHIP mediates polyubiquitination of DNA polymerase beta at 'Lys-41', 'Lys-61' and 'Lys-81', thereby plays a role in base-excision repair. CHIP can also negatively regulate the suppressive function of regulatory T-cells during inflammation by mediating the ubiquitination and degradation of FOXP3 in a HSPA1A/B-dependent manner. CHIP also negatively regulates TGF-beta signaling by modulating the basal levels of SMAD3 via ubiquitin-mediated degradation. Mutations in CHIP cause spinocerebellar ataxia type 16.
25μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2810779 (BioLegend Cat. No. 865701) AB_2810780 (BioLegend Cat. No. 865702)
Structure: CHIP is a 303 amino acid protein with an apparent molecular mass of ~ 35 kD.
Distribution: Tissue Distribution: Brain, heart, skeletal muscle, kidney, liver, and spleen Cellular Distribution: Cytosol, nucleus, and endoplasmic reticulum
Function: CHIP is involved in protein ubiquitination, protein degradation, and neurodegeneration.
Interaction: Ubiquitin, ATXN3, EPHA2, SMAD3, UBE4B, VCP/p97.
Cell Type: Glutamatergic Neurons, Mature Neurons, Neurons, Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neurodegeneration, Neuroscience, Signal Transduction, Ubiquitin/Protein Degradation
Molecular Family: Enzymes and Regulators, Heat Shock Proteins, Postsynaptic proteins, Phospho-Proteins, Protein Modifiers
Antigen References: Zhan S, et al. 2017. Int Rev Immunol. 36(5):300. Cao Z, et al. 2016. Oncotarget. 7(20):29864.
Gene ID: 1027356424287155
UniProt: View information about CHIP on UniProt.org
Clone: W17190B
Regulatory Status: RUO
Other Names: CHIP, STIP1 Homology And U-Box Containing Protein 1, Carboxy Terminus Of Hsp70-Interacting Protein, RING-Type E3 Ubiquitin Transferase CHIP, E3 Ubiquitin-Protein Ligase CHIP, CLL-Associated Antigen KW-8, Antigen NY-CO-7, Heat Shock Protein A Binding
Isotype: Rat IgG2b, κ