Biolegend, 869202, Purified anti-EAAT2 Antibody, 100μg

EAAT2 is the major membrane protein that clears extracellular glutamate from the synaptic area to prevent potential neuronal excitotoxicity from excessive activation of glutamate receptors. EAAT2 is predominantly expressed in the central nervous system on astrocytes and is frequently used as a marker for these cells. Mutations that affect EAAT2 function are clinically associated with amyotrophic lateral sclerosis.
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial recombinant mouse SLC1A2 (EAAT2) protein (a.a. 143 - 238) with GST Tag
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2814631 (BioLegend Cat. No. 869201) AB_2814632 (BioLegend Cat. No. 869202)
Structure: EAAT2 is a 574 amino acid protein with predicted and observed molecular mass of ~ 62 kD.
Distribution: Tissue Distribution: Brain Cellular Distribution: Plasma membrane
Function: EAAT2 is a sodium-dependent amino acid transporter that mediates the uptake of L-Glutamate and L- or D- aspartate.
Interaction: EAAT2 forms homotrimer and interacts with JUB.
Cell Type: Astrocytes
Biology Area: Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family: Presynaptic proteins, Synaptic Vesicle Trafficking/Endocytosis
Antigen References: Pregnolato S, et al. 2019. Front Physiol. 10:417 Parkin GM, et al. 2018. World J Psychiatry. 10.5498/wjp.v8.i2.51 Rosenblum LT, Trotti D. 2017. Adv. Neurobiol. 16:117-136.
Gene ID: 65062051129482
UniProt: View information about EAAT2 on UniProt.org
Clone: A18128E
Regulatory Status: RUO
Other Names: GLT1, SLC1A2, Excitotoxic Amino Acid Transporter 2
Isotype: Mouse IgG2b, κ