Biolegend, 869502, Purified anti-DOPA Decarboxylase (DDC) Antibody, 100μg

DOPA decarboxylase (DDC) is the enzyme that catalyzes several decarboxylation reactions including the conversion of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-Hydroxytryptophan (5-HTP) to serotonin, and L-tryotophan to tryptamine. DDC is the rate-limiting enzyme in dopamine synthesis in patients treated with L-DOPA and in serotonin synthesis in people treated with 5-HTP, such as those affected by Parkinson’ disease and Depression, respectively. Therefore, DDC is an important therapeutic target for these disorders. DDC deficiency is associated with severe developmental delay, oculogyric crises, and autonomic dysfunction.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Human DOPA Decarboxylase (DDC) Protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 µg/ml in human and 0.05 - 10 µg/ml for mouse is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2814635 (BioLegend Cat. No. 869501) AB_2814636 (BioLegend Cat. No. 869502)
Structure: DDC is a 510 amino acid protein with predicted and observed molecular mass of ~54 kD.
Distribution: Tissue Distribution: Kidney, colon, adrenal gland, and brain Cellular Distribution: Cytosol
Function: DDC catalyzes several decarboxylation reactions in vivo including the conversion of DOPA to dopamine and 5-HTP to serotonin.
Interaction: DDC forms Homodimer.
Cell Type: Endothelial cells, Oligodendrocytes
Biology Area: Neurodegeneration, Neuroinflammation, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, Enzymes and Regulators
Antigen References: Himmelreich N, et al. 2019. Mol Genet Metab. 127(1):12 Guenter J, et al. 2016. Postepy Hig Med Dosw. 70(0):1424 Montioli R, et al. 2016. Curr Drug Metab. 17(5):513
Gene ID: 164413195
UniProt: View information about DOPA Decarboxylase on UniProt.org
Clone: A17096A
Regulatory Status: RUO
Other Names: Aromatic-L-Amino-Acid Decarboxylase
Isotype: Mouse IgG2b, κ