Biolegend, 871501, Purified anti-Lamin B2 Antibody, 25μg

Lamin B2 is an essential building block of the nuclear lamina, a filamentous meshwork inside the inner nuclear membrane. It has high degree of protein sequence similarity to another lamin B protein, lamin B1. Both proteins are widely expressed in the early developmental stages of the brain. Knock-out studies have shown that deficiencies in the lamin B proteins lead to defect in neuronal migration. Loss of lamin B2 during retina development prevents proper lamination of adult retinal neurons, impairs synaptogenesis, and reduces cone photoreceptor survival. Lamin B2 is also expressed in the granular component of the nucleolus and functions in modulating nucleolar morphology, dynamics, and ribosome biogenesis.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human Lamin B2 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIHC-P, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 - 10 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 0.5 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2832882 (BioLegend Cat. No. 871501) AB_2832883 (BioLegend Cat. No. 871502)
Structure: Human lamin B2 is a 620 amino acid protein with a predicted molecular mass of 70 kD.
Distribution: Tissue distribution: Ubiquitously expressed Cellular distribution: Nucleus, cytoskeleton
Function: Lamin B2 is a component of nuclear lamina. It provides a framework for the nuclear envelope and interacts with chromatin to regulate gene expression.
Interaction: Interacts with TMEM43
Cell Type: Astrocytes, B cells, Mature Neurons, Microglia, Neurons
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Structure, Chromatin Remodeling/Epigenetics, Neuroscience
Molecular Family: Cytoskeletal Proteins, Nuclear Markers
Antigen References: Wilson KL. 2010. Proc Natl Acad Sci USA. 107:6121-2. Razafsky D, et al. 2016. Mol Biol Cell. 27:1928-37. Lee JM, et al. 2014. Nucleus. 5(4):287-92. Sen Gupta A & Sengupta K. 2017. Mol Cell Biol. e00274-17.
Gene ID: 84823
UniProt: View information about Lamin B2 on UniProt.org
Clone: 8F6-E8-F12
Regulatory Status: RUO
Other Names: Lamin-B2, Lamin B2, LMN2, Epididymis Secretory Sperm Binding Protein, Lamin B3, LAMB2, EPM9
Isotype: Mouse IgG1, κ