Biolegend, 902001, Purified anti-AU5 Epitope Tag Antibody (Previously Covance catalog# MMS-135P), 200microl

The AU5 tag, which consists of the peptide sequence TDFYLK, is often used as a protein modification in order to simplify the labeling and detection of proteins. This unique amino acid sequence allows for specific antibody detection in western blotting and other immunostaining techniques. Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins.
200microl
Verified Reactivity: Epitope tag
Reported Reactivity: Species independent
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: The AU5 monoclonal antibody was raised against an SDS-disrupted BPV-1 preparation and mapped to the six amino acid epitope TDFYLK.
Formulation: Phosphate-buffered solution + 0.03% Thimerosal.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application: WB, ICC, IP, Purification
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. The optimal working dilution should be determined for each specific assay condition. • WB: 1:500* • ICC: 1:500 • IP: 1:150
Application Notes: This antibody is effective in immunofluorescence (IF), immunoblotting, and immunoprecipitation (IP) of tagged proteins. This clone was specifically designed for use in epitope tagging. Because of its small size, clone AU5 is unlikely to alter the activity of the cloned sequence. This antibody was purified using protein-G chromatography.
Application References(PubMed link indicates BioLegend citation): Burke TW, et al. 2001. J Biol Chem. 276:15397. Marinissen MJ, et al. 2001. Genes Dev. 15:535. Lim PS, et al. 1990. J Infect Dis. 162:1263.
Product Citations: Wroblewska A et al. 2018. Cell. 175(4):1141-1155 . PubMed
RRID: AB_2734668 (BioLegend Cat. No. 902007) AB_2565015 (BioLegend Cat. No. 902002)
Biology Area: Cell Biology
Gene ID: NA
UniProt: View information about AU5 on UniProt.org
Clone: AU5
Regulatory Status: RUO
Other Names: AU5 epitope tag, TDFYLK epitope tag, TDFYLK tag
Previously: Covance Catalog# MMS-135P
Isotype: Mouse IgG1