Biolegend, 902602, Purified anti-GFP Epitope Tag Antibody (Previously Covance catalog# MMS-118P), 500microl

Green Fluorescent Protein (GFP) is a single chain 238 amino acid protein derived from the jellyfish Aequoria victoria. GFP emits a bright green fluorescence when illuminated with UV or blue light and is a useful tool for cellular and developmental biologists. Unlike other bioluminescent molecules, GFP does not require additional proteins, substrates or co-factors for its activity.
500microl
Verified Reactivity: Aequorea victoria, Epitope tag
Reported Reactivity: Species independent
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Antibody B34, generated against purified wild type GFP, offers an alternitive for detecting GFP and GFP variants including enhanced/mutant GFP.
Formulation: Phosphate-buffered solution + 0.03% Thimerosal.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application: WB, Purification
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. The optimal working dilution should be determined for each specific assay condition. • WB: 1:5,000*
Application Notes: This antibody is effective in immunoblotting (WB) of GFP labeled proteins. *Predicted MW = 27 kD
Application References(PubMed link indicates BioLegend citation): Pecot MY, Malhotra V. 2004. Cell. 116:99. Kessels MM, et al. 2001. J Cell Biol. 153:351. Gerdes HH, Kaether C. 1996. FEBS Lett. 389:44. Davis I, et al. 1995. Dev Biol. 170:726. Chalfie M. 1995. Photochem Photobio. 62:651. Stearns T. 1995. Curr Biol. 5:262. Chalfie M, et al. 1994. Science. 263:802. Lavy M, et al. 2007. Curr. Biol. 17:947. (WB) Hobl B, et al. 2013. Protein Expr. Purif. 10:32. (WB) Zhang Y, et al. 2007. J. Biol. Chem. 282:13022. (WB) PubMed Asante D, et al. 2013. J. Cell. Sci. 126:5189. (WB) PubMed Oh Y, et al. 2013. Mol Biol Cell. 24:1305. (WB) PubMed Wloka C, et al. 2013. J. Cell. Biol. 200:271. (WB) Redfern RE, et al. 2010. Protein Sci. 19:1948. (WB) PubMed
Product Citations: Eshleman N, et al. 2016. RNA. 22: 1200 - 1214. PubMed Dhingani N, et al. 2020. Sci Rep. 10:18648. PubMed Bhatter N, et al. 2020. Wellcome Open Res. 3:102. PubMed Garnelo Gómez B, et al. 2021. J Cell Sci. 134:. PubMed von Elsner L, et al. 2022. Brain. 145:1551. PubMed Shak C, et al. 2023. J Cell Sci. 133: . PubMed Grochowska KM, et al. 2023. EMBO J. 42:e112453. PubMed Costé de Bagneaux P, et al. 2020. PLoS Genet. 16:e1008625. PubMed Bauer CK, et al. 2019. Am J Hum Genet. 104:1139. PubMed Ulamec SM, et al. 2022. Nat Commun. 13:4986. PubMed Manjunath LE, et al. 2020. J Biol Chem. 295:17009. PubMed Shen K, et al. 2019. Mol Cell. 74:729. PubMed Shearer RF, et al. 2018. Mol Biol Cell. 29:1542. PubMed Herger A, et al. 2020. PLoS Genet. 16:e1008847. PubMed Stehle D, et al. 2018. Sci Rep. 8:12434. PubMed Bhatter N, et al. 2019. FEBS J. 286:4693. PubMed Arthur AL, et al. 2021. Elife. 10: . PubMed Pogorelko G, et al. 2016. PLoS One. 11: 0159757. PubMed Kennedy SP, et al. 2019. Breast Cancer Res. 21:43. PubMed Sánchez-Solana B, et al. 2021. Mol Cancer. 20:141. PubMed Croucher D, et al. 2016. Sci Signal. 9: ra69. PubMed Sánchez-Martín J, et al. 2021. Nat Plants. 7:327. PubMed Borgermann N et al. 2019. The EMBO journal. 38(8) pii: e101496. PubMed Kravchick D, et al. 2016. EMBO J. 35: 1923 - 1934. PubMed Redfern R, et al. 2010. Protein Sci. 19:1948-1956. PubMed Wang Y, et al. 2016. Sci Rep. 6:37213. PubMed Chattopadhyay S, et al. 2022. Nat Commun. 13:7184. PubMed Zhang Y, Barbieri Q 2007. J Biol Chem. 282:13022-13032. PubMed Schmitz F, et al. 2021. Sci Rep. 11:19232. PubMed Basic M, et al. 2021. J Biol Chem. 297:101263. PubMed Liu Y, et al. 2020. Nat Commun. 11:589. PubMed Sakamaki JI, et al. 2017. Mol Cell. 66:517. PubMed Asante D, et al. 2013. J Cell Sci. 126:5189-5197. PubMed Oh Y, et al. 2013. Mol Biol Cell. 24:1305-1320. PubMed Robles‐Valero J et al. 2017. Cancer cell. 32(5):608-623 . PubMed Zhang X, et al. 2016. Nat Commun. 7:12109. PubMed Manjunath LE, et al. 2020. J Biol Chem. 295:17009. PubMed den Brave F, et al. 2020. Cell Rep. 31:107680. PubMed McCaughey J, et al. 2019. J Cell Biol. 218:929. PubMed
RRID: AB_2734671 (BioLegend Cat. No. 902605) AB_2565021 (BioLegend Cat. No. 902601) AB_2565022 (BioLegend Cat. No. 902602)
Biology Area: Cell Biology
Gene ID: NA
UniProt: View information about GFP on UniProt.org
Clone: B34
Regulatory Status: RUO
Other Names: Green Fluorescent Protein
Previously: Covance Catalog# MMS-118P
Isotype: Mouse IgG1