Biolegend, 906103, Biotin anti-6-His Epitope Tag Antibody (Previously Covance catalog# BIOT-156L), 100microl

His tags are commonly used as a method of tagging proteins for detection or purification. This antibody is raised again a polyhistidine tag (6-His or hexa His-tag).
100microl
Verified Reactivity: Epitope tag
Reported Reactivity: Species independent
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Monoclonal 6-His antibody was raised against a recombinant protein containing the sequence HHHHHH.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Previous lots (B435499 and prior) may have been formulated in Phosphate-buffered solution + 0.03% Thimerosal + 50% Glycerol. For further information please contact BioLegend Technical Support or Customer Service.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: WB - Quality tested ICC, IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per mL (1:1000-1:2000 dilution). For immunocytochemistry, a concentration range of 0.5 - 1.0 μg/mL (1:1000-1:2000 dilution) is recommended. For immunoprecipitation, the suggested use of this reagent is 1:150 dilution. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The sequence of the protein flanking the poly His tag does not influence antibody binding. This antibody was subsequently purified via a protein-G column.
Application References(PubMed link indicates BioLegend citation): Deinert K, et al. 2001. J Biol Chem. 276:6000. (WB) Kojima H, et al. 2001. J Biol Chem. 276:19238. (IF) PubMed Lapinski PE, et al. 2000. J Biol Chem. 275:6831. (WB) Bartsch D, et al. 1998. Cell. 95:211. (IF)
Product Citations: Yang LF, et al. 2022. Anal Chem. 94:7278. PubMed Kojima H, et al. 2001. J Biol Chem. 276:19238-19243. PubMed
RRID: AB_2565057 (BioLegend Cat. No. 906103)
Biology Area: Cell Biology
Gene ID: NA
UniProt: View information about 6-His on UniProt.org
Clone: 6-His
Regulatory Status: RUO
Other Names: polyhistidine-tag, hexa histidine-tag, 6xHis-tag, His6 tag, His-tag(TM)
Previously: Covance Catalog# BIOT-156L
Isotype: Mouse IgG1, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.