Biolegend, 906116, Alexa Fluor® 647 anti-6-His Epitope Tag Antibody, 100μg

His tags are commonly used as a method of tagging proteins for detection or purification. This antibody is raised again a polyhistidine tag (6-His or hexa His-tag).
100μg
Verified Reactivity: Epitope tag
Reported Reactivity: Species independent
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Monoclonal 6-His antibody was raised against a recombinant protein containing the sequence HHHHHH.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 2.5 μg/mL (1:200-1:1,000 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: The sequence of the protein flanking the poly His tag does not influence antibody binding. This antibody was subsequently purified via a protein-G column.
Application References(PubMed link indicates BioLegend citation): Deinert K, et al. 2001. J. Biol. Chem. 276:6000. (WB) Kojima H, et al. 2001. J. Biol. Chem.(22)276:19238. (ICC) PubMed Lapinski PE, et al. 2000. J. Biol. Chem. 275:6831. (WB) Bartsch D, et al. 1998. Cell. 95:211. (ICC) Fuentes EJ, et al. 2003. J Biol Chem. 278:2118. PubMed Montegna EA, et al. 2012 PLoS One.7:31156. (WB) PubMed Huang L, et al. 2013. Nat Biotechnol. 31:450. (WB) PubMed
Product Citations: Wong JJ, et al. 2021. Sci Adv. 7:. PubMed
RRID: AB_2750116 (BioLegend Cat. No. 906115) AB_2750117 (BioLegend Cat. No. 906116)
Biology Area: Cell Biology
Gene ID: NA
UniProt: View information about 6-His on UniProt.org
Clone: 6-His
Regulatory Status: RUO
Other Names: polyhistidine-tag, hexa histidine-tag, 6xHis-tag, His6 tag, His-tag(TM)
Isotype: Mouse IgG1, κ