Biolegend, 912501, Purified anti-BG-8 Antibody (Previously Covance catalog# SIG-3317), 1ml

BG-8 is also known as blood group Lewis Y F3. The Lewis Y (type 2 chain) antigen is found on the Type 2 blood group oligosaccharides of glycolipids and glycoproteins. It has been detected in large bowel tumors and colorectal carcinomas. It may be useful in the classification of human renal and bladder tumors and is being investigated as a diagnostic marker for several forms of cancer.
1ml
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This antibody was developed against SK-LU-3 lung cancer cell lines.
Formulation: Phosphate-buffered solution with BSA + 0.09% NaN3.Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation: The antibody was purified by ammonium sulfate precipitation.
Storage & Handling: Store between 2°C and 8°C.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunohistochemical staining. The optimal working dilution should be determined for each specific assay condition. • IHC: ≥1:40 for Biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501). Tissue Sections: Formalin-fixed, paraffin-embedded tissues. Pretreatment: Not required Incubation: 20 minutes at room temperature
Application Notes: This antibody is effective in immunohistochemistry (IHC). Positive tissue (human): Colon adenocarcinoma F3 is specific for Lewis Y (Type 2 Chain).
Application References(PubMed link indicates BioLegend citation): Yaziji H, et al. 2006. Mod Pathol. 19:514. Ordonez NG. 2006. Mod Pathol. 19:34. Ordonez NG. 2006. Mod Pathol. 19:417. Ordonez NG. 2000. Am J Surg Pathol. 24: 598. Zhu ZM, et al. 1995. Biol Reprod. 52:903. Gaffney RA, et al. 1992. J Urol. 148:1341. Jordon D, et al. 1989. Am J Pathol 135:931.
Product Citations: Moya-Gonzalvez EM, et al. 2022. Microbiol Spectr. 10:e0177522. PubMed Rimkute I, et al. 2020. J Biol Chem. 295:15974. PubMed
RRID: AB_2565124 (BioLegend Cat. No. 912501)
Gene ID: NA
UniProt: View information about BG-8 on UniProt.org
Clone: F3
Regulatory Status: RUO
Other Names: Blood Group Related Antigen BG-8
Previously: Signet Catalog# 317-01Covance Catalog# SIG-3317
Isotype: Mouse IgM