Biolegend, 922102, Anti-BG-4 Antibody (Previously Covance catalog# SIG-3313), 1ml

BG-4 is also known as blood group antigen H (type 1).  The H antigen is a precursor to A and B antigens in the ABO system.  The H antigen is a carbohydrate sequence consisting of a chain of β-D-galactose, β-D-N-acetylglucosamine, β-D-galactose, and 2-linked, α-L-fucose, which is linked to protein or ceramide. The H gene locus encodes the fucosyltransferase 1 protein, which is responsible for linking the carbohydrate antigen to the protein.  The H antigen is expressed on erythrocytes and epithelial cells.
1ml
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This antibody was developed against the SW-403 colon cancer cell line. It is specific for Blood group H (type 1).
Formulation: Phosphate-buffered solution with BSA + 0.09% NaN3.Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation: Ammonium sulfate precipitated TCS.
Storage & Handling: Store between 2°C and 8°C.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunohistochemical staining. The optimal working dilution should be determined for each specific assay condition. • IHC: ≥1:40 for Biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501). Tissue Sections: Formalin-fixed, paraffin-embedded tissues. Pretreatment: Not required Incubation: 20 minutes at room temperature
Application Notes: This antibody is effective in immunohistochemistry (IHC). Positive tissue (human): Antrum.
Application References(PubMed link indicates BioLegend citation): Lloyd KO, et al. 1983.Immunogen. 17:537. Sakamoto J, et al. 1984. Mol Immunol. 21:1093. Anger BR, et al. 1982. Hybridoma. 1:139.
Product Citations: Moya-Gonzalvez EM, et al. 2022. Microbiol Spectr. 10:e0177522. PubMed
RRID: AB_2565409 (BioLegend Cat. No. 922102)
Biology Area: Cell Biology
Molecular Family: Blood Group Markers
Gene ID: NA
UniProt: View information about BG-4 on UniProt.org
Clone: 17-206
Regulatory Status: RUO
Other Names: BG4
Previously: Signet Catalog# 313-01Covance Catalog# SIG-3313
Isotype: Mouse IgG3, κ