Biolegend, 933402, Purified anti-Rb Antibody, 100μg

Rb is a key regulator of the cell cycle, more specifically during the transition from the G1 to S phases. The underphosphorylated, active form of Rb interacts directly with E2F1, leading to cell cycle arrest, while the hyperphosphorylated form decouples from E2F1, thus promoting the transcription of genes promoting entry into the S phase. Rb is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human Rb protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IP, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/mL. For immunocytochemistry, a concentration of 5 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2820205 (BioLegend Cat. No. 933401) AB_2820206 (BioLegend Cat. No. 933402)
Structure: Rb is a 928 amino-acid protein with a predicted molecular weight of 110 kD.
Distribution: Localized to the nucleoplasm/Ubiquitously expressed.
Function: Regulator; tumor suppressor; chromatin stability
Interaction: E2F1 Transcription Factor
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Cycle/DNA Replication
Molecular Family: Tumor Suppressors
Antigen References: Dunham A, et al. 2004. Nature. 428(6982):522-8. Hogg A, et al. 1992. Oncogene. (7):1445-51. Cho HS, et al. 2012. Neoplasia. 14(6):476-86. Mori T, et al. 2011. Cell Cycle. 10(19):3284-99. Rose JE, et al. 2010. Mol Med. 16(7-8):247-53.
Gene ID: 5925
UniProt: View information about Rb on UniProt.org
Clone: W18116A
Regulatory Status: RUO
Other Names: RB Transcriptional Corepressor 1, Retinoblastoma-Associated Protein, Protein Phosphate 1, Regulatory Subunit 130
Isotype: Rat IgG2a, κ