Biolegend, 933602, Purified anti-GLUD1 Antibody, 100μg

Glutamate dehydrogenase (GLUD) is a mitochondrial enzyme involved in the reversible oxidative deamination of glutamate to ammonia and α-ketoglutarate (α-KG), which is a key intermediate in the tricarboxylic acid (TCA) cycle. α-KG is utilized to produce ATP, which in turn increases the ATP/ADP ratio and induces insulin secretion. GLUD mutations are associated with hyperinsulinism-hyperammonemia (HI/HA) syndrome. Humans have two isoenzymes, GLUD1 and GLUD2, each encoded by distinct genes. GLUD1 is widely expressed while GLUD2 is expressed in neural and testicular tissues.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human GLUD1 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone recognizes both human and mouse GLUD1.Methanol permeabilization is recommended for ICC testing. Triton X-100 permeabilization showed weak signal.
RRID: AB_2820209 (BioLegend Cat. No. 933601) AB_2820210 (BioLegend Cat. No. 933602)
Structure: GLUD1 is a 558 amino acid protein with a predicted molecular weight of 61 kD.
Distribution: Mitochondrial matrix/ubiquitously expressed
Function: Glutaminolysis
Biology Area: Cell Biology, Mitochondrial Function
Molecular Family: Enzymes and Regulators
Antigen References: Stanley CA, et al.1998. N Engl J Med. 338:1352-7 Plaitakis A, et al. 2000. J Neurochem. 75:1862 Yang C, et al. 2009. Cancer Res. 69:7986 Jin L, et al. 2015. Cancer Cell. 27:257
Gene ID: 2746
UniProt: View information about GLUD1 on UniProt.org
Clone: W18177A
Regulatory Status: RUO
Other Names: Glutamate Dehydrogenase 1, EC 1.4.1.3, GLUD, Epididymis Tissue Sperm Binding Protein Li 18mP, Epididymis Secretory Sperm Binding Protein, GDH 1
Isotype: Rat IgG2b, κ