Biolegend, 934101, Purified anti-HMOX1 Antibody, 25μg

HMOX1 is a member of the heme oxygenase family. Unlike its constitutive counterpart, it is an inducible enzyme triggered by external stimuli such as nitric oxide, growth factors, heavy metals, UV radiation, phorbol esters, inflammatory cytokines, heme, etc. Transcription of HMOX1 in tissue has been shown to be a product of the nrf2/ARE pathway where an increase in its protein levels results in heme degradation. The tissue protective effects of HMOX1 are a result of the bioactive products produced after heme degradation: iron ions, carbon monoxide, and biliverdin which is converted to bilirubin via biliverdin reductase.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IP, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 - 0.5 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone was tested for ICC using three fix/perm methods (PFA/Triton-X, PFA/MeOH, MeOH only), the best method was the use of PFA/Triton X-100. In the CoCl2 treated cells for ICC, staining was seen in less than 5% of cells. Clone was tested for ICFC using cytokine fix/perm method only (based on ICC results).
RRID: AB_2894520 (BioLegend Cat. No. 934101) AB_2894520 (BioLegend Cat. No. 934102)
Distribution: Cytosol/Endoplasmic reticulum
Biology Area: Cell Biology
Molecular Family: Enzymes and Regulators
Antigen References: 1. Yang B, et al. 2018. Toxicol Appl Pharmacol. 355:189-197 2. Chora A, et al. 2007. J Clin Invest. 117(2):438-47 3. Harding H, et al. 2003. Mol Cell. 11(3):619-33.
Gene ID: 3162
UniProt: View information about HMOX1 on UniProt.org
Clone: W19398C
Regulatory Status: RUO
Other Names: Heme Oxygenase 1, HO-1, Heat Shock Protein, 32-KD
Isotype: Rat IgG2a, κ