Biolegend, 934104, PE anti-HMOX1 Antibody, 100tests

HMOX1 is a member of the heme oxygenase family. Unlike its constitutive counterpart, it is an inducible enzyme triggered by external stimuli such as nitric oxide, growth factors, heavy metals, UV radiation, phorbol esters, inflammatory cytokines, heme, etc. Transcription of HMOX1 in tissue has been shown to be a product of the nrf2/ARE pathway where an increase in its protein levels results in heme degradation. The tissue protective effects of HMOX1 are a result of the bioactive products produced after heme degradation: iron ions, carbon monoxide, and biliverdin which is converted to bilirubin via biliverdin reductase.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone was tested for ICC using three fix/perm methods (PFA/Triton-X, PFA/MeOH, MeOH only), the best method was the use of PFA/Triton X-100. In the CoCl2 treated cells for ICC, staining was seen in less than 5% of cells. Clone was tested for ICFC using cytokine fix/perm method only (based on ICC results).
RRID: AB_2941636 (BioLegend Cat. No. 934103) AB_2941636 (BioLegend Cat. No. 934104)
Distribution: Cytosol/Endoplasmic reticulum
Biology Area: Cell Biology
Molecular Family: Enzymes and Regulators
Antigen References: 1. Yang B, et al. 2018. Toxicol Appl Pharmacol. 355:189-197 2. Chora A, et al. 2007. J Clin Invest. 117(2):438-47 3. Harding H, et al. 2003. Mol Cell. 11(3):619-33.
Gene ID: 3162
UniProt: View information about HMOX1 on UniProt.org
Clone: W19398C
Regulatory Status: RUO
Other Names: Heme Oxygenase 1, HO-1, Heat Shock Protein, 32-KD
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.