Biolegend, 934701, Purified anti-CDC20 Antibody, 25μg

CDC20 is a co-activator of the anaphase-promoting complex or cyclosome (APC/C) and is a cell cycle regulator that initiates the metaphase-to-anaphase transition. In early mitosis, APC/C is phosphorylated by Cdk1 and CDC20 binds to the APC/C. CDC20 activates the APC/C-mediated ubiquitination of anaphase inhibitors, including securin and cyclin B. Degradation of these proteins results in the activation of separase, which cleaves the Scc1 subunit of cohesion complex to trigger chromosome segregation, and thereby promoting mitotic exit.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human CDC20 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone does not recognize mouse CDC20.This clone recognized a ~75 kD protein of unknown origin by western blot and immunoprecipitation. Because of this, we have not tested or recommend this clone for immunocytochemistry.Due to the presence of the ~75 kD protein of unknown origin in W18156A immunoprecipitates, we do not recommend the clone to be used for co-immunoprecipitation applications.
RRID: AB_2820217 (BioLegend Cat. No. 934701) AB_2820218 (BioLegend Cat. No. 934702)
Structure: CDC20 is a 499 amino acid protein with a predicted molecular weight of 55 kD.
Distribution: Centrosome/ubiquitously expressed
Function: Coactivator/Cell cycle regulator
Biology Area: Cell Cycle/DNA Replication
Antigen References: Visintin R, et al. 1997. Science. 278: 460. Fang G, et al. 1998. Mol. Cell. 2:163. Shirayama M, et al. 1999. Nature. 402:203. Yu H. 2007. Mol. Cell. 27:3. Pesin JA and Orr-Weaver TL. 2008. Annu. Rev. Cell. Dev. Biol. 24:475. Izawa D and Pines J. 2011. Nat Cell Biol. 13:223
Gene ID: 991
UniProt: View information about CDC20 on UniProt.org
Clone: W18156A
Regulatory Status: RUO
Other Names: Cell Division Cycle 20, Cell Division Cycle Protein 20 Homolog, BA276H19.3, CDC20A, BA276H19.3, p55CDC
Isotype: Rat IgG2a, κ