Biolegend, 934901, Purified anti-EED Antibody, 25μg

Embryonic ectoderm development (EED) is a member of the polycomb-group (PcG) family. The PcG family members form multimeric protein complexes called polycomb repressive complexes (PRCs). These polycomb repressive complexes (PRCs) epigenetically silence gene expression and are involved in a variety of biological processes such as cell cycle control, stem cell plasticity, cellular senescence and cancer. There are multiple isoforms (~4), which seem to interact differently with different PRCs. Isoform 1 of EED also has an alternate phosphorylated form. Diseases associated with EED include Cohen-Gibson syndrome and Weaver syndrome.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human EED protein of approximately 250 amino acids
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIP, ICC, KO/KD-WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone recognizes 3 isoforms of human and mouse EED.For ICC testing, HeLa cells were fixed in 4% PFA and permeabilized with either Triton X-100 or methanol; both methods produced strong staining at 1 mg/mL.
RRID: AB_2820221 (BioLegend Cat. No. 934901) AB_2820222 (BioLegend Cat. No. 934902)
Structure: EED is an ~440 amino acid protein with 4 isoforms of a predicted molecular weight of around 50 kD.
Distribution: EED mainly localizes to the nucleoplasm.
Function: Maintains transcriptionally repressive state of genes.
Interaction: EZH2
Biology Area: Cancer Biomarkers, Cell Cycle/DNA Replication, Cell Death, Chromatin Remodeling/Epigenetics, Stem Cells
Antigen References: Van der Vlag J, et al. 1999. Nat. Gen. 23:474. Martin C, et al. 2006. J. Bol. 281:13:8365. Cao Q, et al. 2014. Nat. Comm. 5:3127. Kuzmichev A, et al. 2004. Mol. Cell. 14:183. Vire E, et al. 2005. Nat. 439:871. Schuettengruber B, et al. 2009. Development. 136: 353.
Gene ID: 8726
UniProt: View information about EED on UniProt.org
Clone: W18151A
Regulatory Status: RUO
Other Names: Embryonic Ectoderm Development, Embryonic Ectoderm Development Protein, Polycomb Protein EED
Isotype: Rat IgG2a, κ