Biolegend, 935003, Ultra-LEAF™ Purified anti-human Neurturin Antibody, 100μg

Neurturin belongs to the GDNF family of ligands, which consists of 4 members: GDNF, Neurturin, Artemin, and Persephin. GDNF family members orchestrate multiple aspects of the development and maintenance of the central and peripheral nervous systems. These GDNF family ligands signal through a multicomponent receptor system composed of a common signaling component RET (receptor tyrosine kinase) and one of the high affinity binding coreceptors GFRα (α1-α4). Neurturin is a GDNF family member that functions as a ligand for the GFRα2 and RET complex. Neurturin is a potent neurotrophic factor that can promote the survival of numerous neuronal populations, which include the sympathetic, nodose, and dorsal root ganglion sensory neurons. Neurturin is synthesized as a precursor protein that is cleaved to release the carboxy-terminal domain. The carboxy-terminal domain contains seven conserved cysteine residues that are necessary for the formation of the cysteine-knot and the single interchain disulfide bond. The functional form of human Neurturin is a disulfide-linked homodimer of the carboxy-terminal 102 amino acid residues. Studies have shown positive results for Neurturin in protecting and repairing dopaminergic neurons in various models of Parkinson's disease. In association with mutations of the RET gene, genetic variations in Neurturin may contribute to Hirschsprung's disease.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human Neurturin
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Block - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by blocking the binding between recombinant human GFRα2 and recombinant human Neurturin (Cat. No. 760304). The ND50 range is 0.2 - 5 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2861117 (BioLegend Cat. No. 935003) AB_2861118 (BioLegend Cat. No. 935004)
Structure: Disulfide-linked homodimer
Distribution: Neurturin is a secreted protein that is expressed in both neuronal tissues and non-neuronal tissues such as the pancreas, heart, and hair follicles.
Function: It is a neurotrophic factor and promotes the survival of sympathetic, nordose, and dorsal root ganglion sensory neurons.
Interaction: Neurturin targets multiple central and peripheral neurons including the midbrain dopaminergic, sympathetic, and sensory neurons.
Ligand/Receptor: Interacts with GDNF family receptor α-2 (GFRα2). Has a lower affinity with GFRα1.
Cell Type: Embryonic Stem Cells, Mature Neurons, Neural Stem Cells, Neurons
Biology Area: Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family: Growth Factors
Antigen References: Kotzbauer PT, et al. 1996. Nature 6608:467-70. Doray B, et al. 1998. Hum. Mol. Genet. 7:1449-52. Milbrandt J, et al. 1998. Neuron 20:245-53. Baloh RH, et al. 1998. Neuron. 21:1291-302. Sariola H, Saarma M. 2003. 116:3855-3862. Ruiz-Ferrer M, et al. 2011. J. Mol. Med. (Berl) 5:471-80. Lindholm D, et al. 2016. Cell Mol. Life Sci. 7:1365-79.
Gene ID: 4902
UniProt: View information about Neurturin on UniProt.org
Clone: A16100E
Regulatory Status: RUO
Other Names: NRTN, NTN
Isotype: Mouse IgG2b, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.