Biolegend, 935201, Purified anti-IMPDH1 Antibody, 25μg

Inosine-5'-monophosphate dehydrogenase 1 (IMPDH1) was initially identified as an oncogene in several human cancers. IMPDH1 catalyzes the conversion of inosine-5'-monophosphate (IMP) to xanthosine-5'-monophosphate (XMP) and is the first committed rate-limiting step in the de novo synthesis of GTP, and consequently it is involved in the regulation of cell growth. IMPDH1 is also part of the composition of the cytoplasmic rod and ring structures (RR) and is involved in maintaining cellular nucleotide pools needed for DNA and RNA synthesis.
25μg
Verified Reactivity: Human, Mouse, Hamster
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length IMPDH purified from a Chinese hamster V79 cell variant
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IP, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone recognizes human, mouse and hamster IMPDH1 and is predicted to recognize the rodent orthologs. Clone is likely to cross react with IMPDH2. For ICC testing, HeLa cells were fixed in 4% PFA and permeabilized with either Triton X-100 or methanol; both methods produced strong staining at 1 µg/mL.
RRID: AB_2832890 (BioLegend Cat. No. 935201) AB_2832891 (BioLegend Cat. No. 935202)
Structure: IMPDH1 is an 514 aminoacid protein with a predicted molecular weight of 56 kD.
Distribution: Localized to the cytosol filamentous rods and rings / Ubiquitously expressed
Function: Regulation of cell growth, and biosynthesis of de novo GTP
Biology Area: Cell Cycle/DNA Replication, Cell Proliferation and Viability, DNA Repair/Replication
Antigen References: Calise SJ, et al. 2015. Front Immunol. 6:41. Liao LX, et al. 2017. Proc Natl Acad Sci U S A. 29. Duan S, et al. 2018. J Exp Clin Cancer Res. 37:304. Zhao Y, et al. 2018. Biomarkers. 1:9. Carcamo WC, et al. 2011. PLoS One. 6. Xu Y, et al. 2017. Sci Rep. 7:745. Glesne DA, et al. 1991. Mol Cell Biol. 11:5417-25. Hager PW, et al. 1995. Biochem Pharmacol. 49:1323-9.
Gene ID: 3614
UniProt: View information about IMPDH1 on UniProt.org
Clone: 1D6-E3
Regulatory Status: RUO
Other Names: Inosine Monophosphate Dehydrogenase 1, Inosine-5'-Monophosphate Dehydrogenase 1, IMP Dehydrogenase 1, IMPD1, IMP (Inosine Monophosphate) Dehydrogenase 1, Inosine Monophosphate Dehydrogenase Type 1, IMPDH 1
Isotype: Mouse IgG2b, κ