Biolegend, 936001, Purified anti-PER1 Antibody, 25μg

PER1 (Period Circadian Regulator 1), a transcriptional repressor, is a component of the circadian clock and a member of the Period family. The circadian clock is the body’s internal time keeping mechanism, which regulates various physiological processes including hormone rhythms, locomotor activity, sleep cycle, and behavior. PER1 is expressed throughout peripheral tissues but expression is most significant in the suprachiasmatic nucleus (SCN) region of the brain which functions as the primary circadian pacemaker in mammals. PER1 is expressed in an oscillating rhythm with a period of about 24 hours which is maintained even in constant darkness. PER1 expression is light dependent, with exposure to light during subjective night increasing expression and shifting its phase in the SCN. Full length PER1 localizes to the cytoplasm with a shorter nuclear isoform localizing in the nucleus. Diseases associated with PER1 include periodic limb movement disorder (PLMD), advanced sleep phase syndrome, cardiovascular diseases and cancer.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human PER1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IP, KO/KD-WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.0625 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone recognizes human and mouse PER1. Clone is not recommended for ICC.
Product Citations: Tampakakis E, et al. 2021. Sci Adv. 7:eabh4181. PubMed
RRID: AB_2832904 (BioLegend Cat. No. 936001) AB_2832905 (BioLegend Cat. No. 936002)
Structure: PER1 is a 1290 amino acid protein with a predicted molecular weight of 136 kD.
Distribution: PER1 is mainly localized to the nucleus, with additional localization to the cytosol.
Function: Component of the circadian clock
Interaction: TIMELESS, PER2, PER3, CRY1 and CRY2
Ligand/Receptor: NR1D1/2 and RORA/B/G
Cell Type: Neurons
Biology Area: Neuroscience
Antigen References: Gery S, et al. 2006. Mol Cell. 22:3:375. Chen S, et al. 2005. Carcinogenesis. 26:7:1241. Yan L, et al. 1999. Neuroscience. 94:1:141. Shigeyoshi Y, et al. 1997. Cell. 91:7:1043.
Gene ID: 5187
UniProt: View information about PER1 on UniProt.org
Clone: W17208B
Regulatory Status: RUO
Other Names: Period Circadian Regulator 1, Period Circadian Protein Homolog 1, Circadian Clock Protein PERIOD 1, Period Circadian Clock 1, RIGUI, HPER1, PER, Circadian Pacemaker Protein RIGUI, Circadian Pacemaker Protein Rigui
Isotype: Rat IgG2a, κ