Biolegend, 937001, Purified anti-GM130 Antibody, 25μg

GM130 is a structural cis-Golgi protein that plays an essential role in vesicular transport. Mitotic fragmentation of the Golgi apparatus occurs through the CDK1-dependent regulation of the physical interaction between GM130 and the vesicle docking protein p115. Biochemical or genetic disruption of GM130 results in defective Golgi assembly. GM130 also plays a role in activating TPX2, a spindle assembly factor, to produce microtubule nuclei surrounding the Golgi, which are then directly bound by GM130 during mitotic events. Aberrant GM130 expression has been described in multiple malignancies where cells show altered Golgi structure and loss of polarity, lending to increased migration and invasion.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human GM130 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone does not recognize mouse GM130 by western blot. This clone was tested for ICC using HeLa cells fixed with 4% PFA and permeabilized with Triton X-100 or methanol. Both methods were compatible with GM130 staining.
RRID: AB_2888895 (BioLegend Cat. No. 937001) AB_2888895 (BioLegend Cat. No. 937002)
Distribution: Cis-Golgi/Ubiquitously expressed
Function: Golgi structural protein
Biology Area: Cell Biology, Cell Structure
Molecular Family: Microtubules, Organelle Markers
Antigen References: 1. Nakamura N, et al. 1997. Cell. 89:445. 2. Lowe M, et al. 1998. Cell. 94:783. 3. Wei JH, et al. 2015, Cell. 162:1.
Gene ID: 2801
UniProt: View information about GM130 on UniProt.org
Clone: W18248A
Regulatory Status: RUO
Other Names: Golgin A2, Golgin subfamily A member 2, 130 kD cis-Golgi matrix protein, Golgin 95, GOLGA2, SY11 Protein
Isotype: Rat IgG2b, κ