Biolegend, 937802, Purified anti-COX IV Antibody, 100μg

COX IV is a subunit of cytochrome C oxidase (COX), a critical component of the mitochondrial respiratory pathway. COX is an enzyme comprised of multiple subunits which work to remove electrons from cytochrome C and transfer them to molecular oxygen, this action also promotes proton pumping from the mitochondrion matrix to the intermembrane space to maintain electrochemical stability. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic human COXIV peptide
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC, IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1 µg/mL. For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone was tested for ICC using 4% PFA-fixed HeLa cells permeabilized with either methanol or Triton X-100. While staining was observed with both methods, methanol permeabilization resulted in stronger staining.
RRID: AB_2876756 (BioLegend Cat. No. 937801) AB_2876756 (BioLegend Cat. No. 937802)
Structure: COX IV is a 169 amino acid protein with a predicted molecular weight of 19 kD.
Distribution: Mitochondria
Biology Area: Cell Biology, Mitochondrial Function
Molecular Family: Enzymes and Regulators
Antigen References: Capaldi RA. 1990. Annu Rev Biochem. 59:569. Sharma V, et al. 2015. Proc Natl Acad Sci U S A. 112:2040-5. Li Y, et al. 2006. J Bioenerg Biomembr. 38:283-91.
Gene ID: 1327
UniProt: View information about COX IV on UniProt.org
Clone: 4D11-B3-E8
Regulatory Status: RUO
Other Names: COX4I1, Cytochrome C Oxidase Subunit 4I1, Cytochrome C Oxidase Subunit 4 Isoform 1, Mitochondrial, Cytochrome C Oxidase Subunit IV Isoform 1, Cytochrome C Oxidase Polypeptide IV, COX IV-1, COX4
Isotype: Mouse IgG1, κ