Biolegend, 938002, Purified anti-BCAT1 Antibody, 100μg

BCAT1 is an aminotransferase that catalyzes the catabolism of the branched-chain amino acids leucine, isoleucine, and valine, making it an important regulator of the TCA cycle. BCAT1 also plays a role in cell cycle progression, differentiation, and apoptosis. Clinical disorders involved with mutations in the BCAT1 gene include hypervalinemia and hyperleucine-isoleucinemia.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Full-length recombinant human BCAT1 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone failed to immunoprecipitate BCAT1 from NTERA-2 lysates. This clone was tested for immunocytochemistry on NTERA-2 cells fixed with 4% PFA and permeabilized with either methanol or Triton X-100. Both methods resulted in dim staining of BCAT1. We therefore do not recommend the clone for this application.
RRID: AB_2876757 (BioLegend Cat. No. 938001) AB_2876757 (BioLegend Cat. No. 938002)
Structure: BCAT1 is a 386 amino acid protein with a predicted molecular weight of 43 kD.
Distribution: Ubiquitously expressed/Cytosolic
Function: Aminotransferase enzyme, amino acid catabolism
Cell Type: Macrophages
Biology Area: Cell Biology, Mitochondrial Function
Molecular Family: Enzymes and Regulators
Antigen References: Papathanassiu AE, et al. 2017. Nat. Commun. 8:16040. Xu M, et al. 2016. Oncol Lett. 12:2648-2656. Zhou W, et al. 2013. Mol Cancer. 12:53. Wegermann K, et al. 2018. PLoS One. 13:e0204308. Hattori A, et al. 2017. Nature. 545:500-504.
Gene ID: 586
UniProt: View information about BCAT1 on UniProt.org
Clone: W18024A
Regulatory Status: RUO
Other Names: Branched Chain Amino Acid Transanimase 1, BCT1, BCATC, PNAS121, Protein ECA39
Isotype: Rat IgG2a, κ