Biolegend, 938301, Purified anti-RAP1 Antibody, 25μg

Repressor/activator protein 1 (RAP1), also called telomeric repeat-binding factor 2-interacting protein 1 (TERF2IP), is one of six subunits of the shelterin complex, which regulates telomerase activity and protects telomeric DNA through the generation of t-loops. RAP1 forms a heterodimer with shelterin subunit TRF2 and regulates telomere length, recombination, and transcription. This complex also functions in the repression of homology-directed repair (HDR) and prevents telomere deletions. In the cytoplasm, RAP1 functions as an inhibitor kinase (IKK) adaptor and modulator of the nuclear factor- κB (NF-κB) pathway as well as an activator of NF-κB transcriptional activity. TERF2IP S205 phosphorylation has been implicated in the regulation of activation, apoptosis, and senescence of endothelial cells.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human RAP1 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IP, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. For immunocytochemistry, a concentration of 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone does not recognize mouse RAP1. ICC testing was conducted on Raji cells fixed with 4% paraformaldehyde and permeabilized with either Triton X-100 or methanol. Both methods were compatible with RAP1 staining.
RRID: AB_2876760 (BioLegend Cat. No. 938301) AB_2876760 (BioLegend Cat. No. 938302)
Structure: RAP1 is a 399 amino acid protein with an expected molecular weight of 55 kD.
Distribution: Ubiquitously expressed/Nuclear
Function: Transcription, Transcription regulation
Biology Area: Cell Biology, DNA Repair/Replication
Antigen References: Cai Y, et al. 2017. Cell Cycle. 16:1765-1773. Kotla S, et al. 2019. JCI Insight. 4(9):124867. Rai R, et al. 2015. Nat Commun. 7:10881. Zhang Y, et al. 2015. Cell Death Discov. 1:15007.
Gene ID: 54386
UniProt: View information about RAP1 on UniProt.org
Clone: W18011A
Regulatory Status: RUO
Other Names: TERF2IP, TERF2 Interacting Protein, TERF2-interacting telomeric protein 1, TRF2-interacting telomeric protein 1, Repressor/activator protein 1 homolog, Dopamine receptor-interacting protein 5, DRIP5
Isotype: Rat IgG2b, κ