Biolegend, 938402, Purified anti-Vinculin Antibody, 100μg

Vinculin is a filamentous actin (F-actin) binding protein that was originally described as a critical component of focal adhesion plaques. It plays an important role in the transmission of mechanical forces, stabilization of cell-cell junctions via a direct interaction with α-catenin, and linking of integrins to the actin cytoskeleton. Loss of Vinculin protein expression contributes to metastatic phenotypes in cancer tissue due to loss of cell-cell adhesion. Vinculin has been isolated from prostate cancer exosomes and may serve as a biomarker for the progression of taxane-resistance prostate cancers; it is also a common marker for cell-cell and focal adhesion junctions.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human Vinculin protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IP, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone was tested for ICC using HeLa cells fixed with 4% PFA and permeabilized with Triton X-100 or methanol. While methanol permeabilization was compatible with Vinculin staining, Triton X-100 permeabilization resulted in dim staining. We therefore do not recommend Triton X-100 as a permeabilization method. While this clone passed IP testing, it failed to quantitatively enrich Vinculin from whole cell extracts and may not be suitable for detecting transient or low-abundance protein-protein interactions by co-immunoprecipitation. This clone is predicted to recognize Metavinculin due to complete sequence homology between the immunogen and the corresponding region of Metavinculin.
RRID: AB_2876761 (BioLegend Cat. No. 938401) AB_2876761 (BioLegend Cat. No. 938402)
Structure: Vinculin is a 1,066 amino acid protein with a predicted molecular weight of 116 kD. Metavinculin is a 1,134 amino acid protein with a predicted molecular weight of 124 kD.
Distribution: Ubiquitously expressed/cytosol, cytoskeleton, and focal adhesion plaques; Metavinculin expression is restricted to smooth skeletal muscle tissue.
Function: Cell-matrix and cell-cell adhesion
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure
Molecular Family: Cytoskeletal Proteins
Antigen References: Kawakami K, et al. 2015. Int J Oncol. 47:384-90. Geiger B, et al. 1980. Proc Natl Acad Sci U S A. 77:4127-31. Jockusch BM, et al. 1981. Proc Natl Acad Sci U S A. 78:3005-9.
Gene ID: 7414
UniProt: View information about Vinculin on UniProt.org
Clone: W18245A
Regulatory Status: RUO
Other Names: Metavinculin, MV, Epididymis Secretory Sperm Binding Protein, Epididymis Luminal Protein 114
Isotype: Rat IgG2a, κ