Biolegend, 939201, Purified anti-NRF2 Antibody, 25μg

NRF2 is a transcription factor that plays a critical role in inducing expression of genes required for oxidative stress defense and stress balance. It does so by binding to antioxidant response elements (AREs), which are located upstream of target genes. NRF2 is degraded by ubiquitination of KEAP1 E3 ligase. In a clinical setting, NRF2 is frequently activated in many types of cancers; it drives metabolic adaptation and survival in ROS-rich tumor microenvironments.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human NRF2 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IP, IHC-P, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. For immunohistochemistry, a concentration range of 5.0 - 20.0 µg/mL is suggested. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone was tested for ICC on 4% PFA-fixed HeLa cells and permeabilized with either methanol or Triton X-100. Both permeabilization methods were compatible with NRF2 staining. Clone W19086B is validated to detect NRF2 in mouse NIH/3T3 cells using a Western blot on whole cell lysate.
Product Citations: Gupta A, et al. 2022. JCI Insight. . PubMed
RRID: AB_2892502 (BioLegend Cat. No. 939201) AB_2892502 (BioLegend Cat. No. 939202)
Structure: NRF2 is a 605 amino acid protein with a predicted molecular weight of 67 kD.
Distribution: Ubiquitously expressed/Nucleus
Function: Transcription factor/Redox balance
Biology Area: Cell Biology, Mitochondrial Function, Transcription Factors
Antigen References: M. da Costa R, et al. 2019. Front In Pharm. 10:3389. Shoemaker A. 2017. Sci Trans Med. 9:420. Robledinos-Antón N, et al. 2019. Hindawi. 10:1155. Cuadrado A, et al. 2019. Nat Rev Drug Discov. 18:295-317.
Gene ID: 4780
UniProt: View information about NRF2 on UniProt.org
Clone: W19086B
Regulatory Status: RUO
Other Names: Erythroid 2 Like 2, NF-E2-Related Factor 2, HEBP1, IMDDHH
Isotype: Rat IgG2a, κ