Biolegend, 939208, Alexa Fluor® 488 anti-NRF2 Antibody, 100tests

NRF2 is a transcription factor that plays a critical role in inducing expression of genes required for oxidative stress defense and stress balance. It does so by binding to antioxidant response elements (AREs), which are located upstream of target genes. NRF2 is degraded by ubiquitination of KEAP1 E3 ligase. In a clinical setting, NRF2 is frequently activated in many types of cancers; it drives metabolic adaptation and survival in ROS-rich tumor microenvironments.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human NRF2 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: This clone was tested for ICC on 4% PFA-fixed HeLa cells and permeabilized with either methanol or Triton X-100. Both permeabilization methods were compatible with NRF2 staining. Clone W19086B is validated to detect NRF2 in mouse NIH/3T3 cells using a Western blot on whole cell lysate.
RRID: AB_3675199 (BioLegend Cat. No. 939207) AB_3675199 (BioLegend Cat. No. 939208)
Structure: NRF2 is a 605 amino acid protein with a predicted molecular weight of 67 kD.
Distribution: Ubiquitously expressed/Nucleus
Function: Transcription factor/Redox balance
Biology Area: Cell Biology, Mitochondrial Function, Transcription Factors
Antigen References: M. da Costa R, et al. 2019. Front In Pharm. 10:3389. Shoemaker A. 2017. Sci Trans Med. 9:420. Robledinos-Antón N, et al. 2019. Hindawi. 10:1155. Cuadrado A, et al. 2019. Nat Rev Drug Discov. 18:295-317.
Gene ID: 4780
UniProt: View information about NRF2 on UniProt.org
Clone: W19086B
Regulatory Status: RUO
Other Names: Erythroid 2 Like 2, NF-E2-Related Factor 2, HEBP1, IMDDHH
Isotype: Rat IgG2a, κ